基于果实相关性状的江西野生毛花猕猴桃初级核心种质的构建方法研究  

Study on the method of constructing a primary core collection of Jiangxi wild Actinidia eriantha based on fruit traits

作　　者：郎彬彬 黄春辉[1] 朱博[1] 谢敏[1] 张文标[1] 钟敏[1] 曲雪艳[1] 陶俊杰[1] 徐小彪[1] 

机构地区：[1]江西农业大学农学院,南昌330045

出　　处：《果树学报》2016年第7期794-803,共10页Journal of Fruit Science

基　　金：江西省科技厅重大科技专项(20143ACF60015);江西省教育厅落地计划(KJLD14026);国家自然科学基金(31360472;31460505);国家科技基础性工作专项(2012FY110100-7)

摘　　要：【目的】利用在江西省南城县、宜黄县境内收集的118份野生毛花猕猴桃资源果实的20个表型性状,研究野生毛花猕猴桃初级核心种质的构建方法。【方法】采取逐步聚类,在30%取样比例下,以欧式距离为遗传距离,对3种取样方法(随机取样、偏离度取样和优先取样)、4种系统聚类方法(类平均法、离差平方和法、最长距离法和最短距离法)进行比较,以筛选出的最佳构建策略进一步比较不同取样比例(15%、20%、25%、30%、35%及40%)的构建效果,从而确定最适取样比例。【结果】(1)比较3种取样方法表明,采用优先取样法优于其他2种取样方法;(2)比较4种聚类方法表明,类平均法优于其他3种聚类方法;(3)最适取样比例为30%。【结论】通过主成分分析法验证表明,在30%取样比例下,采取欧式距离,利用类平均法进行逐步聚类,结合优先取样法构建的野生毛花猕猴桃初级核心种质能够最大程度地保留原始种质的遗传信息。[ Objective ] Actinidia eriantha Benth. is the unique kiwifruit germplasm resource in China, and Jiangxi province is one of the main distribution areas. The researches about the fruit traits and the genetic diversity of germplasm resources of wild A. eriantha have been previously reported, but there have been almost no relevant reports about the construction of core collection resources of A. eriantha published so far. In this study, 118 wild A. eriantha resources were collected from Nancheng and Yihuang counties in Jiangxi province, and proper methods were determined utilizing 20 fruit phenotypic traits to construct the primary core collection resources of wild A. eriantha. [Methods] A total of 118 germplasm were collected from 3 scattered areas with similar growing environments around the Magu mountain nature protection area of Nancheng county in Jiangxi province （N27°31′46″, E116°32′52″ to N27°32′00″, E116°32′ 16″） The experiment was carried out from September 2013 to December 2014, and the experimental materials were collected by stages （Nov. 1-Nov. 10）. The standard time for collection was achieved when the soluble solid contents （SSC） reached 6.0%. The materials ripened and softened naturally in the laboratory at room temperature, then 20 fruit traits were chosen to be observed and analyzed according to DUS testing stan- dards （Actinidia）. The 20 fruit traits included fruit weight, fruit shape index, edible rate, relative core size, peel strength, dry matter content, flesh color, the viscosity of pulp, peel hardness, ascorbic acid （AsA）, titratable acid, soluble sugar, soluble solid contents （SSC）, color of core, cross section of fruit shape, cross section of core shape, fruit shape, rostral end shape, fruit shoulder shape, and fruit hair density. All the traits were measured when fruits reached an edible state. The quality traits were assigned according to the standards of description. The quantity trait was standardized with the distance of 2/3 standard deviatio

关 键 词：野生毛花猕猴桃 初级核心种质 取样方法 聚类方法 取样比例 主成分分析 

分 类 号：S663.4[农业科学—果树学]