柔毛水杨梅对人微血管内皮细胞增殖、迁移、成管能力的影响  

Effect of Geum japonicum Thunb. var. chinense F. Bolle on Proliferation,Migration and Tube Formation of Human Microvascular Endothelial Cells in Vitro

作　　者：刘城[1] 宋雨鸿[1] 王月刚[1] 裴静娴[1] 赖艳娴[1] 申艳[1] 

机构地区：[1]广州市第一人民医院心血管内科,广东广州510180

出　　处：《广州中医药大学学报》2016年第4期551-555,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81100235)

摘　　要：【目的】研究柔毛水杨梅对人微血管内皮细胞增殖、迁移、成管能力的影响。【方法】将不同浓度（0~200 ng/m L）柔毛水杨梅作用于人微血管内皮细胞（HMVEC-Ls）,分别采用MTS法检测细胞的增殖活性,划痕法检测细胞的迁移能力,Matrigel法检测细胞的成管能力以及Western blot法验证低氧诱导因子-1α（HIF-1α）、血管内皮生长因子（VEGF）、端粒酶逆转录酶（TERT）的表达水平。【结果】MTS结果显示：柔毛水杨梅与HMVEC-Ls共同培养7 d,25~200 ng/m L不同浓度组均可促进HMVEC-Ls增殖,与对照组（0 ng/m L）比较,差异均有统计学意义（P〈0.05）,以100 ng/m L组促进HMVEC-Ls增殖能力最显著（P〈0.05）。划痕实验结果显示：与对照组比较,50~150 ng/m L组HMVEC-Ls迁移率显著增大,差异均有统计学意义（P〈0.05）,以150 ng/m L组HMVEC-Ls迁移率最高。成管实验结果：与对照组比较,50~150 ng/m L组HMVECLs成管长度显著增加,差异均有统计学意义（P〈0.05）,亦以150 ng/m L组HMVEC-Ls成管长度最大。Western blot检测结果：与对照组比较,150 ng/m L柔毛水杨梅浓度组HIF-1α、TERT和VEGF表达均显著升高（P〈0.05）。【结论】柔毛水杨梅可通过促进人微血管内皮细胞增殖、迁移、管样形成而发挥促血管新生效应,其机制可能与HIF-1α-TERT-VEGF途径介导的血管新生有关。Objective To study the effects of Geum japonicum ThuMb. var. chinense F. Bolle （GJ） on the proliferation, migration and tube formation of human microvascular endothelial cells （HMVEC-Ls） in vitro. Methods The HMVEC-Ls were treated with different concentrations of GJ methanol extract. HMVEC-Ls proliferation, migration and tube formation were measured by MTS assay, wound-healing cell migration assay and Matrigel assay, respeetively. The effect of GJ methanol extract on the expression levels of hypoxia inducible factor 1 α（ HIF- 1α）, telomerase reverse transcriptasc （TERT） and vascular endothelial growth factor（ VEGF ）were detected by Western blotting method. Results MTS results showed that HMVEC-Ls proliferation index in 25- 200 ng/mL of GJ groups was increased（P〈0.05 as compared with that of the control group） , the effect of 100 ng/mL of GJ group being the best. HMVEC-Ls migration ratio in 50-150 ng/mL of GJ groups was enhanced（P〈 0.05 as compared with that of the control group）, the effect of 150 ng/mL of GJ group being the best. The tube length of HMVEC-Ls in 50-150 ng/mL of GJ groups was enhanced （P〈0.05 as compared with that of the control group）, the effect of 150 ng/mL of GJ group being the best. In addition, HIF-1α, TERT and VEGF protein expression levels were also significantly up-regulated by GJ （P〈0.05）. Conclusion GJ can promote HMVEC-Ls proliferation, migration and tube formation, and the mechanism is related with angiogenesis mediated by HIF-1α-TERT-VEGF pathway.

关 键 词：柔毛水杨梅 人微血管内皮细胞 血管新生 细胞培养 

分 类 号：R285.5[医药卫生—中药学]