根瘤菌共生对低温胁迫下紫花苜蓿抗寒生理变化的影响  

Effect of Symbiotic Rhizobium in Alfalfa on Physiological Change Under Cold Stress

作　　者：李莎莎[1] 张志强[1] 王亚芳[1] 张攀[1] 曹玉曼[1] 杨培志[1] 呼天明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《草地学报》2016年第2期377-383,共7页Acta Agrestia Sinica

基　　金：国家自然基金(31272490;31372357);农业部公益性行业专项(201403048-3);国家"十二五"支撑计划(2011BAD17B05);中央基本科研业务费(QN2013026);国家牧草产业技术体系(CARS-35)资助

摘　　要：为研究根瘤菌共生对紫花苜蓿（Medicago sativa）在低温胁迫下抗寒生理指标变化的影响,对低温胁迫下接种和不接种根瘤菌的紫花苜蓿的地上部分及地下部分的相对电导率、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、脯氨酸、可溶性蛋白、可溶性糖等指标进行测定。结果显示：低温胁迫下,根瘤菌共生紫花苜蓿的相对电导率（-6℃,4-6h）显著低于不接种紫花苜蓿（P〈0.05）,且其MDA含量低于不接种紫花苜蓿;根瘤菌共生紫花苜蓿的SOD比活力、CAT活性、POD活性以及脯氨酸、可溶性蛋白、可溶性糖含量均高于不接种根瘤菌紫花苜蓿。根瘤菌共生紫花苜蓿在低温胁迫下具有更强的膜稳定性、活性氧防御以及渗透调节能力,具有更强的抗寒能力。Low temperature is one of the important environment factors that can limit alfalfa（Medicago sativa）in the growth,development and distribution.To understand whether symbiotic rhizobium in alfalfa affect the physiological changes under cold stress,we analyzed the change of relative conductivity,methane dicarboxylic aldehyde（MDA）,activities of superoxide dismutase（SOD）,catalase（CAT）,peroxidase（POD）,proline content,total soluble protein and soluble sugar in alfalfa under cold stress.Results showed that the relative conductivity and the content of MDA were lower in the alfalfa with active nodules（NA）than in the alfalfa without nodules（NN）.The SOD and CAT and POD activities,proline content,soluble protein and soluble sugar contents were significantly higher in NA than in NN under cold stress.The results suggested NA may have higher cell membrane stability,antioxidative capability and osmoregulatory capacity.NA may have stronger cold resistance ability.

关 键 词：紫花苜蓿 根瘤菌共生 低温胁迫 生理指标 

分 类 号：S541[农业科学—作物学]