邓恩桉下胚轴不定芽再生体系建立的影响因素  

Factors Affecting System Establishment of Adventitious Bud Regeneration from Hypocotyls of Eucalyptus dunnii

作　　者：陆荣生[1] 韩美丽[1] 梁志强[1] 覃建林[1] 

机构地区：[1]广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,广西南宁530007

出　　处：《江西农业学报》2016年第6期31-35,共5页Acta Agriculturae Jiangxi

基　　金：广西自然科学基金资助项目(2013GXNSFAA019074);广西作物病虫害生物学重点实验室资助项目(13-051-47-KF-3)

摘　　要：以邓恩桉种子萌发的实生苗下胚轴为材料,研究了不同激素种类与浓度及多胺对下胚轴经愈伤组织阶段产生不定芽过程的影响,以建立一种下胚轴不定芽再生快繁体系。结果表明,以MS为基本培养基,在培养基中添加2,4-D 1.0-1.5 mg/L可诱导邓恩桉下胚轴产生高质量的愈伤组织;愈伤组织增殖的适宜培养基为MS＋TDZ 0.5-1.0 mg/L,愈伤分化产生不定芽的适合培养基为MS＋TDZ 1.0 mg/L＋腐胺15-25 mg/L,愈伤不定芽分化率达77.1%-79.2%,且外植体褐化率得以降低。愈伤组织块移入分化培养基初期,3-7 d暗培养可使愈伤不定芽分化率提高到82.8%以上。The hypocotyls from 10-day-old aseptic seedling,were used as explants to investigate the influence of callus formation and adventitious buds differentiation in media adding different types of hormone and ploy-amine,in order to establish a rapid propagation regeneration system of hypocotyls by adventitious bud. The results showed that high quality callus were obtained from hypocotyls in medium with basal MS salt contained 2,4-D 1.0 - 1.5 mg / L. The medium with basal MS salt adding TDZ 0.5 - 1.0 mg /L,were most suitable for callus proliferation. The optimal medium for adventitious buds differentiation,was basal MS salt supplemented TDZ 1.0 mg / L and putrescine 15 - 25 mg / L,and 77.1% - 79.2% of adventitious buds differentiation rate were achieved in this medium,meanwhile browning rate of explants were alleviated. Darkness culture for 3 - 7 d at the early stage of callus subculture,could make adventitious buds differentiation rate increase to 82.8%.

关 键 词：邓恩桉 下胚轴 不定芽 愈伤 分化 

分 类 号：Q813.12[生物学—生物工程]