针刺康复疗法影响脑性瘫痪模型大鼠大脑病变组织生长相关蛋白43和突触小泡蛋白的表达  

Effects of acupuncture and rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin at the injury site of cerebral palsy rats

作　　者：侯玉晋 康豪鹏 

机构地区：[1]河南中医学院第二附属医院儿童脑病康复科,河南省郑州市450002 [2]河南电力医院内一科,河南省郑州市450052

出　　处：《中国组织工程研究》2016年第27期3999-4005,共7页Chinese Journal of Tissue Engineering Research

基　　金：河南省高等学校重点科研项目计划(15A360003)~~

摘　　要：背景:针刺联合康复治疗是目前脑性瘫痪最有效的治疗方法,生长相关蛋白43和突触小泡蛋白是神经发育以及突触密度的特征性标记物,参与神经细胞的生长、修复、再生以及突触重塑。目的:观察针刺联合康复疗法对脑性瘫痪大鼠大脑病变组织生长相关蛋白43和突触小泡蛋白表达的影响,探讨针刺联合康复疗法治疗脑性瘫痪的机制。方法:将125只大鼠随机分为5组:假手术组、模型组、康复组、针刺组、针刺康复组。后4组均建立脑性瘫痪模型,假手术组和模型组不予治疗处理,康复组进行康复训练,针刺组给予针刺治疗,针刺康复组给予康复训练和针刺治疗。结果与结论:(1)运动功能:针刺康复组模后第3-5周的运动功能评分优于模型组、康复组和针刺组(P<0.05);(2)生长相关蛋白43和突触小泡蛋白的表达:免疫组织化学染色显示,与模型组相比,康复组、针刺组和针刺康复组在第2-4周时大鼠大脑病变组织生长相关蛋白43的表达,建模后4,5周突触小泡蛋白的表达均升高(P<0.05),其中针刺康复组均升高最明显(P<0.05)。(3)结果表明,针刺联合康复疗法促进脑性瘫痪大鼠大脑病变组织生长相关蛋白43和突触小泡蛋白的表达,以此发挥神经保护作用。BACKGROUND： Acupuncture ＋ rehabilitation therapy is the most effective treatment for cerebral palsy. Growth associated protein-43 and synaptophysin were characteristic markers for neurodevelopment and synaptic density, and involved in nerve cell growth, repair, regeneration and synapse remodeling. OBJECTIVE： To study the effects of acupuncture ＋ rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin in cerebral palsy rats and to investigate the mechanism of acupuncture ＋ rehabilitation therapy on cerebral palsy. METHODS： The 125 rats were randomly divided into five groups： sham group, model group, rehabilitation group, acupuncture group, and acupuncture ＋ rehabilitation group. Rat models of cerebral palsy were established in model group, rehabilitation group, acupuncture group, and acupuncture ＋ rehabilitation group. Rat models in the sham and model groups did not receive treatment. Rat models in the rehabilitation group underwent rehabilitation training. Rat models in the acupuncture group were subjected to acupuncture. Rat models in the acupuncture ＋ rehabilitation group underwent rehabilitation training and acupuncture. RESULTS AND CONCLUSION：（1） Motor function： The motor function scores were better in the acupuncture ＋ rehabilitation group than in the model, acupuncture, and rehabilitation groups（P〈0.05）.（2） Growth associated protein-43 and synaptophysin expression： immunohistochemical staining revealed that compared with the model group, growth associated protein-43 expression at 2-4 weeks and synaptophysin expression at 4 and 5 weeks were higher in the acupuncture and acupuncture ＋ rehabilitation groups（P〈0.05）; the increase in above expression was most significant in the acupuncture ＋ rehabilitation group（P〈0.05）.（3） Results confirmed that acupuncture combined with rehabilitation promoted growth associated protein-43 and synaptophysin expression in rats with cerebral palsy and exerted neuroprotective effect.

关 键 词：组织工程 生长相关蛋白43 模型 动物 实验动物 神经损伤与修复动物模型 针刺 康复训练 针康法 针刺治疗 脑性瘫痪 大鼠 突触小泡蛋白 运动功能 

分 类 号：R318[医药卫生—生物医学工程]