丰富环境对血管性痴呆模型大鼠梨状皮质未成熟神经元表达的影响  

Effects of enriched environment on immature neurons in piriform cortex of a rat model of vascular dementia

作　　者：黄俊[1] 吴帆帆 贺旭[2,3] 李建平[4] 严小新[3] 潘爱华[3] 李志远[3] 

机构地区：[1]邵阳医学高等专科学校解剖教研室,湖南省邵阳市422000 [2]益阳医学高等专科学校解剖教研室,湖南省益阳市413000 [3]中南大学湘雅医学院人体解剖与神经生物学系,湖南省长沙市410013 [4]遵义医学院人体解剖学系,贵州省遵义市563000

出　　处：《中国组织工程研究》2016年第27期4006-4012,共7页Chinese Journal of Tissue Engineering Research

基　　金：湖南省教育厅课题(13C958);2015年湖南省大学生研究性学习和创新性试验计划项目~~

摘　　要：背景:研究表明丰富环境可改善血管性痴呆大鼠的认识功能障碍,但是目前国内外关于其作用机制的报道较少。目的:从行为学水平观察丰富环境对血管性痴呆大鼠认知功能的影响,并探讨其作用机制。方法:采用永久性结扎两侧颈总动脉方法建立血管性痴呆模型,将痴呆大鼠分成血管性痴呆组(n=8)和丰富环境组(n=12)。血管性痴呆组置于常规饲养环境里饲养30 d,丰富环境组进行丰富环境刺激30 d。运用Morris水迷宫检测两组大鼠的认知功能,免疫组织化学和免疫印迹技术检测两组大鼠梨状皮质微管相关蛋白(DCX,未成熟神经元的标记物)阳性细胞数目和蛋白水平,免疫荧光观察微管相关蛋白与NeuN(神经元特异性核抗原)共表达细胞的数目。结果与结论:(1)血管性痴呆组逃避潜伏期显著长于丰富环境组(P<0.05),跨越平台次数少于丰富环境组,差异有显著性意义(P<0.05);(2)血管性痴呆组梨状皮质微管相关蛋白阳性细胞数目和微管相关蛋白水平与丰富环境组相比,均显著下降(P<0.05);(3)血管性痴呆组梨状皮质微管相关蛋白与NeuN的共表达细胞显著低于丰富环境组(P<0.05);(4)结果提示,丰富环境可能通过促进梨状皮质未成熟神经元的表达及分化而改善血管性痴呆大鼠的认知功能。BACKGROUND： Researches showed that the enriched environment could improve the cognitive dysfunction of rats with vascular dementia. However, there are few reports regarding its mechanism of action. OBJECTIVE： To explore the effect of enriched environment on the cognitive dysfunction of rats with vascular dementia from the behavioral level. METHODS： Vascular dementia models were made by permanent ligation of bilateral common carotid arteries and were divided into vascular dementia group（n=8） and enriched environment group（n=12）. Vascular dementia group was taken care under conventional breeding environment for 30 days, while the enriched environment group was subjected to the enriched environment for 30 days. Morris water maze test was adapted to test the cognitive function of rats between two groups. Immunohistochemistry and immunoblotting were applied to observe the number of DCX~＋ cells and DCX protein level in both groups. The number of DCX-labeled cells co-expressing NeuN was observed using immunofluorescence technique. RESULTS AND CONCLUSION：（1） The escape latency in the vascular dementia group was longer than that in the enriched environment group（P〈0.05）. The times across the platform was less in the vascular dementia group than that in the enriched environment group（P〈0.05）.（2） In comparison with the enriched environment group, the number of DCX-positive cells and its protein level in the piriform cortex were significantly decreased in the vascular dementia group（P〈0.05）.（3） The number of DCX/NeuN co-labeled cells in the piriform cortex was significantly less in the vascular dementia group than in the enriched environment group（P〈0.05）.（4） These findings suggested that enriched environment could improve the cognitive dysfunction of rats with vascular dementia through promoting the expression and differentiation of the immature neurons.

关 键 词：模型 动物 痴呆 血管性 环境医学 组织工程 实验动物 神经损伤与修复动物模型 丰富环境 血管性痴呆 大鼠 认知 梨状皮质 未成熟神经元 

分 类 号：R318[医药卫生—生物医学工程]