Genetic Diversity Analysis and Fingerprint Construction of Major Mango Cultivars in China  

我国芒果主栽品种遗传多样性分析及指纹图谱构建（英文）

作　　者：王明[1] 应东山[1] 王琴飞[1] 李莉萍[1] 张如莲[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部热带作物品种资源利用重点开放实验室,海南儋州571737

出　　处：《Agricultural Science & Technology》2016年第6期1289-1294,共6页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Hainan Province(34128);Fundamental Scientific Research Funds of Chinese Academy of Tropical Agricultural Sciences(1630032013031)~~

摘　　要：[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding of mango. [Method] With 115 pairs of SSR primers, genetic diversity analysis and cluster analysis were performed for 30 mango cultivars, among which the genetic relationships were analyzed. [Result] Total 64 pairs of polymorphic primers were screened out from the 115 pairs of primers, and total 343 bands were amplified from the 30 cultivars with 73.2% of polymorphic bands. On average, 3.9 allelic loci were detected for each pair of primers with genetic diversity index of 0.5, Shannon＇s diversity index of 1.00 and polymorphism information content of 0.49, indicating higher genetic diversity. The cluster analysis showed that the 30 major cultivars could be classified into four categories. The first category included 14 cultivars; the second category included 11 cultivars, most of which were introduced from abroad; the third category included 4 cultivars, Le., Miansan, Parayinda, Baiyu and Hongxiangya： the fourth category included only one cultivar Maqiesu.By using 7 pairs of SSR markers, i.e., M42, M49, M54, M55, M96, M99 and M103, digital fingerprints were constructed for the 30 mango cultivars. [Conclusion] The 30 mango cultivars present more complex genomic genetics and abundant genetic information, and they have higher genetic diversity.[目的]研究芒果主栽品种遗传多样性,构建指纹图谱,为芒果品种创新及选育提供理论依据。[方法]利用115对SSR引物对30个芒果品种进行遗传多样性分析和聚类分析,分析其亲缘关系。[结果]从115对SSR引物中筛选出64对多态性引物,扩增条带总数为343条,多态性比例为73.2%。每对引物可检测到等位位点3.9个,基因多样性为0.54,Shannon’s信息指数为1.00,多样性信息平均指数为0.49,具有较高的遗传多样性。聚类分析结果表明,可将30份主栽品种分为四大类,第Ⅰ类包括14份材料;第Ⅱ类包括11份材料,主要为国外引种而获得的品种;第Ⅲ类包括4份材料,为缅三、帕拉英达、白玉和红象牙;第Ⅳ类仅有一份材料,为马切苏。选用7对SSR标记M42、M49、M54、M55、M96、M99和M103构建了30份芒果种质的数字化指纹图谱,可完全区分30份芒果主栽品种。[结论]30份芒果材料的基因组遗传较复杂,遗传信息较丰富,具有较高的遗传多样性。

关 键 词：MANGO Major cultivar SSR marker Cluster analysis FINGERPRINT 

分 类 号：S667.7[农业科学—果树学]