五种细菌侵染后韭菜迟眼蕈蚊体内防御反应的研究  

Defensive respondes of Bradysia odoriphaga to inoculation of five species of bacteria

作　　者：高欢欢[1] 翟一凡[1] 徐楠 覃冬云[1] 葛温伯 于毅[1] 

机构地区：[1]山东农业科学院植物保护研究所,济南250100 [2]山东省产品质量检验研究院,济南250102 [3]青岛农业大学植物保护学院,山东青岛266109

出　　处：《环境昆虫学报》2016年第3期582-588,共7页Journal of Environmental Entomology

基　　金：社会公益性研究项目(201303027)

摘　　要：为了解韭蛆体内5种细菌对韭菜迟眼蕈蚊Bradysia odoriphaga的影响,本研究将解淀粉芽孢杆菌Bacillus amyloliquefaciens、居泉沙雷氏菌Serratia fonticola、恶臭假单胞杆菌Pseudomonas putida、枯草芽胞杆菌Bacillus subtilis和节杆菌Arthrobacter nitroguajacolicus 5种细菌通过浸泡和针刺两种方式对韭蛆进行侵染,观察韭蛆存活率、蛋白含量以及3种抗氧化酶活性的变化规律。结果显示,与浸泡接种细菌相比,针刺接种细菌后,韭蛆整体的存活率和SOD酶活性下降,而蛋白含量、POD活性和CAT活性上升。其中,居泉沙雷氏菌在针刺侵染后对韭蛆表现出致病性。因此,机械损伤对于细菌的侵染具有重要的作用,韭蛆则可通过改变抗氧化酶的活性来防御细菌的侵染。本研究为探索韭蛆与体内共生微生物之间的关系提供重要的基础,为韭蛆的共生防治提供指导。In order to understand the effect of bacteria on Bradysia odoriphaga,the survival rate,protein content and three oxidative enzymes of B. odoriphaga were examined after inoculation by five species of bacteria according to two inoculation methods（ soak and wound）. The bacteria were： Bacillus amyloliquefaciens, Serratia fonticola, Pseudomonas putida, Bacillus subtilis and Arthrobacter nitroguajacolicus. The result showed that,compared to soak inoculation by bacteria,survival rate and SOD activity of B. odoriphaga decreased,and protein content,activity of POD and CAT increased after wound inoculation. The negative influence on B. odoriphaga was found after wound inoculation by S. fonticola. It was concluded that,could be affected by the mechanical injury could contributed to the infection of bacteria to B. odoriphaga. B. odoriphaga could produced defensive responds to the inoculation of bacteria through changing activity of oxidative enzymes. This study provided the theoretical foundation for exploring the coevolution relationships between B. odoriphaga and microorganisms. And we hope that it will contribute to guiding for the symbiotic control of B. odoriphaga.

关 键 词：韭蛆 细菌 侵染 保护酶 

分 类 号：Q965.8[生物学—昆虫学] S436.3[农业科学—农业昆虫与害虫防治]