大白菜光周期调控因子CCA1的差异分析及功能标记开发  

Differential Analysis and Functional Marker Development of Photoperiod Regulation Factor CCA1 from Two Chinese Cabbage Inbred Lines

作　　者：张志刚[1] 刘栓桃[1] 李巧云[1] 王晓[1] 王立华[1] 赵智中[1] 王淑芬[1] 徐文玲[1] 刘贤娴[1] 刘辰[1] 

机构地区：[1]山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100

出　　处：《山东农业科学》2016年第6期1-6,181,共6页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2014CM021;ZR2015YL073);山东省农业科学院青年基金项目(2014QNM11);山东省农业良种工程项目"十字花科名产蔬菜新品种选育"(2014LNK-96-PZTP-10)

摘　　要：本研究利用春季和秋季条件下光周期的变化趋势,对两个大白菜自交系06-247与He102的光周期敏感性进行了鉴定,结果是06-247对长日照敏感,而He102对长日照不敏感。采用同源克隆技术,比较分析两材料光周期响应关键调控因子CCA1的全长c DNA序列,发现二者编码区存在两处6 bp插入/缺失和14处非同义SNPs差异;二者编码产物分别包含552、556个氨基酸残基;其非同义SNPs造成不同氨基酸的替换;二者间氨基酸序列差异主要位于CCA1蛋白中间区域的蛋白-蛋白相互作用结构域和C端的磷酸化修饰结构域。本研究开发出区分两处6 bp插入/缺失的共显性分子标记。该结果为深入研究CCA1在大白菜光周期响应调节过程中的功能奠定了基础。By the trend of photoperiod in spring and autumn,the sensibilities of two Chinese cabbage inbred lines,06- 247 and He102,to photoperiod were identified. The 06- 247 was sensitive to long day,while He102 was opposite. The full- length c DNA sequence of photoperiod regulation factor CCA1（ Circadian clock associated 1） from two lines were compared by homology- based cloning. It was revealed that there were two 6- bp In Dels and 14 non- synonymous SNPs between the CCA1 coding regions of 06- 247 and He102. The coding product had 552 and 556 amino acid residues respectively. The non- synonymous SNPs caused the exchange of amino acids with different property. The amino acid differences were mainly located in middle protein- protein interaction domain and C- terminal phosphorylation domain. The codominant molecular markers to distinguish the two 6- bp In Dels were developed and validated. The results provided foundation for further elucidating the molecular mechanism of photoperiodic response of CCA1 in Chinese cabbage.

关 键 词：大白菜 依赖/不依赖长日照开花型 BraCCA1 插入/缺失突变 功能标记 

分 类 号：S634.103.6[农业科学—蔬菜学]