T细胞表位多肽疫苗对禽流感病毒的免疫保护评价  

Immune Protection Evaluation of T Cell Epitope Peptide Vaccine for Avian Influenza Virus

作　　者：朱凤珠 鲁梅[3] 谭刘刚 孔正杰 黄庆华[1] 黄艳艳[1] 杨少华[1] 张秀美[1] 崔言顺[2] 许传田[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]山东农业大学动物科技学院,山东泰安271018 [3]山东潍坊工程职业学院,山东青州262500

出　　处：《山东农业科学》2016年第6期105-108,共4页Shandong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(201303033);现代农业产业技术体系(CARS-42-Z12)

摘　　要：为了探究NP蛋白T细胞表位多肽NP_(67-74)-KLH对流感通用疫苗4M2e-MAP免疫效果的影响,本研究将多肽NP_(67-74)-KLH和4M2e-MAP添加完全弗氏佐剂和不完全弗氏佐剂后免疫BALB/c小鼠,通过攻毒保护试验来评价免疫效果。攻毒以后小鼠体重变化曲线说明,联合免疫多肽疫苗NP_(67-74)-KLH和4M2e-MAP的试验组小鼠体重变化趋势比单一免疫4M2e-MAP合成肽疫苗更加趋于平稳,并且联合免疫试验组小鼠体重恢复较快。荧光定量和肺部组织病理切片结果均表明,复合多肽(NP_(67-74)-KLH和4M2eMAP)能在一定程度上干扰流感病毒在脏器肺中的复制并能够降低病毒对肺部的损伤程度,在抵抗病毒过程中可以产生有效保护。该研究为多肽疫苗联合应用以抵抗流感研发提供了较好思路。To explore the effect of T cell epitope peptide NP_（67-74）- KLH of NP protein on immune effect of 4M2e- MAP,the BALB / c mice were immunized by NP_（67-74）- KLH and 4M2e- MAP after adding the complete freund＇s adjuvant and incomplete freund ＇s adjuvant to evaluate the immune effect. The weight change curve of mice after challenge showed that the weight change trend of mice immunized combinedly by NP_（67-74）- KLH and 4M2e- MAP was more smooth than that immunized by 4M2e- MAP,and the weight of mice in combined immunization group regained quickly. The results of fluorescent quantitation and lung tissue pathological slices showed that the compound polypeptide of NP_（67-74）- KLH with 4M2e- MAP could inhibit the virus replication in the lungs,reduce the pathological damage to lungs and get the effective protection during the course of resisting virus. This provided a good reference for the combined use of peptide vaccine against avian influenza research.

关 键 词：禽流感 多肽疫苗 免疫效果 

分 类 号：S852.45[农业科学—基础兽医学]