利用SSR分子标记鉴定玉米杂交种‘吉祥1号’纯度  

Identification of Maize Hybrid Varieties Using SSR Marker

作　　者：车卓 常宏 张伟 

机构地区：[1]甘肃省种子管理局,兰州730020

出　　处：《中国农学通报》2016年第18期28-32,共5页Chinese Agricultural Science Bulletin

基　　金：甘肃省陇原青年创新人才扶持计划"我省玉米杂交种纯度DNA分子鉴定标准研制"(2015-67)

摘　　要：纯度是种子质量的重要指标。‘吉祥1号’玉米杂交种是在甘肃省选育并大面积栽培的主推玉米品种。为鉴定该品种的纯度,本研究开展了‘吉祥1号’纯度鉴定SSR标记方法研究。通过比较快速提取法和改良CTAB法2种DNA提取方法在SSR分子标记纯度鉴定中的应用,结果表明:2种方法提取的DNA均适用于SSR分子标记纯度检测,但快速提取法具有简单、快速的特点,更适用于分子标记纯度鉴定。从20对SSR引物中筛选出2个标记bnlg2291k4和bnlg2305k4,用于‘吉祥1号’品种的纯度鉴定,2个标记均能明显的区分亲本和杂交种。在‘吉祥1号’杂交种中人为混入双亲,采用本研究确定的方法可准确鉴定出混入的自交系。Purity is an important parameter of seed quality.‘Jixiang No 1＇is a main maize hybrid variety andwidely cultivated in Gansu Province. To identify the purity of the variety, the authors conducted a research onSSR purity identification method of‘Jixiang No 1＇. Through the comparison of rapid extraction method andimproved CTAB method used in the purity test of‘Jixiang No 1＇with SSR marker, the results showed that theDNA extracted by the above two methods was applicable to the purity detection of SSR molecular markers, butthe rapid extraction method had the characteristics of simple, fast, and was more suitable for the purityidentification. bnlg2291k4 and bnlg2305k4 were selected from 20 pairs of SSR markers for purity test of‘Jixiang No 1＇, both markers could clearly distinguish parents and hybrids. Mixing parents seeds into hybridseeds of‘Jixiang No 1＇, the method established by this study could clearly identify inbred lines.

关 键 词：玉米杂交种 '吉祥1号’ SSR分子标记 纯度鉴定 

分 类 号：S513[农业科学—作物学]