母乳中生长抑素及胃动素含量变化与变态反应相关性研究  

Research of relations between allergy and change of levels of somatostatin and motilin in breast milk

作　　者：谢咏梅[1] 高珊[1] 王丽媛[1] 汪志凌[1] 蔡晓唐[1] 周晖[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041

出　　处：《中华妇幼临床医学杂志（电子版）》2016年第3期260-265,共6页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家自然科学基金面上项目(81170607)~~

摘　　要：目的探讨母乳中生长抑素(SST)及胃动素(MTL)含量变化与婴儿摄入母乳发生变态反应的相关性,旨在为进一步研究婴儿食物摄入导致变态反应的机制提供帮助。方法选择2011年1月至2015年12月于四川大学华西第二医院消化儿科住院治疗,并且被确诊为因摄入母乳而发生变态反应的23例患儿为研究对象,纳入研究组。患儿摄入母乳发生变态反应的诊断标准为:纯母乳喂养患儿发生反复消化道症状,停止母乳摄入后症状消退,母乳再激发试验呈阳性。本研究组纳入标准:因消化道症状反复多次就诊的<6个月纯母乳喂养婴儿,符合母乳摄入导致变态反应的诊断标准。排除标准:消化道畸形、先天遗传性疾病及特殊病原体消化道感染者。按照随机数字表法随机选择同期于本院接受常规儿童保健的纯母乳喂养健康婴儿23例,纳入对照组。采集研究组与对照组受试者大便标本及摄入母乳标本,采用放射免疫测定(RIA)检测标本中生物活性成分SST与MTL含量,并进行统计学比较。研究组与对照组受试者性别构成比、年龄、体重及摄入母乳情况等比较,差异均无统计学意义(P>0.05)。本研究遵循的程序符合四川大学华西第二医院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试者监护人的知情同意,并与之签署临床研究知情同意书。结果两组受试者摄入母乳中生物活性成分SST含量:研究组为(236.7±28.9)pg/mg,对照组为(412.6±36.7)pg/mg,研究组摄入母乳中SST含量显著低于对照组,并且差异有统计学意义(t=2.821,P=0.012);摄入母乳中MTL含量:研究组为(159.4±36.7)pg/mg,对照组为(216.8±59.7)pg/mg,研究组摄入母乳中MTL含量显著低于对照组,并且差异亦有统计学意义(t=2.517,P=0.026)。两组受试者大便标本中SST含量:研究组为(32.6±8.9)pg/mg,对照组为(56.2±12.7)pg/mg,研究组大便标本中SST含量显著低于对照�Objective To study the relations between allergy and change of somatostatin（SST）and motilin（MTL）in breast milk,so as to provide reference for further research of pathomechanism of food allergy in infants.Methods From January 2011 to December 2015,a total of 23 cases of infants who were diagnosed as allergic gastroenteritis because of pure breast feeding were included into research group.All the patients were hospitalized in Department of Gastrointestinal in West China Second University Hospital,Sichuan University.Diagnostic criteria for allergy of breast milk were repeated alimentary symptoms which would release when stop breast feeding,and rechallenge test of breast milk induced the same symptoms.Inclusion criteria for allergic gastroenteritis infants in research group included：younger than 6months of exclusive breastfeeding infants who visited medical clinic frequently as digestive disorders,and exclusion criteria were alimentary malformation,hereditary diseases and gastrointestinal infection of specific pathogen.During the same time and in the same hospital,a total of 23 cases of normal infants with pure breast feeding were collected as control group.Samples of their breast milk and stools were collected in two groups.Levels of SST and MTL in breast milk and stools were detected by radioimmunoassay（RIA）.There were no significant differences between two groups among gender ratios,age and weight,breast-feeding practices,etc.（P〉0.05）.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of West China Second University Hospital,Sichuan University.Informed consent was obtained from the parents of each participating infant.Results Level of SST in breast milk was（236.7±28.9）pg/mg in research group,and（412.6±36.7）pg/mg in control group,level of SST in research group was much lower than that in control group,and there was significant difference between two groups（t=2.821,P=0.012）.Level of MTL in breast milk was（159.4±36.7）pg/mg in research gro

关 键 词：母乳喂养 腹泻 婴儿 变态反应和免疫学 生长抑素 促胃动素 

分 类 号：R725.9[医药卫生—儿科]