组胺H4受体在过敏性紫癜患者外周血及肾脏组织中的表达  

Expression of Histamine H4 Receptor in Peripheral Blood Mononuclear Cells and Kidney of HSP Patient

作　　者：雷蕾[1] 蒋瑾瑾[1] 周霖[1] 顾军[1] 

机构地区：[1]第二军医大学附属长海医院,上海200433

出　　处：《中国中西医结合皮肤性病学杂志》2016年第3期133-135,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：上海市卫计委青年基金项目;编号:20134y194

摘　　要：目的比较过敏性紫瘢(HSP)患者外周血及肾脏组织H4R的表达情况,初步探讨H4R在HSP中作用。方法收集126名初次发病HSP患者及94名健康体检者外周血标本,利用逆转录-聚合酶链反应(RT-PCR)方法,分别比较HSP急性期组与健康对照组之间、HSP急性期不同临床表型之间H4RmRNA的表达情况。收集HSP肾脏损害患者肾活检标本及健康肾组织标本,利用免疫组化比较H4R的表达情况。结果 HSP急性期患者外周血单个核细胞表面H4R mRNA表达水高于健康对照组(P<0.01)。肾脏受累组外周血单个核细胞表面H4R的表达明显高于单纯皮肤组、非肾病混合组(P<0.01)。肾脏受累组肾脏组织中H4R表达呈阳性,以肾小管为主,阳性率明显高于健康对照组(P<0.01)。结论 H4R可能参与了HSP的急性期发病,且与HSP患者的肾脏受损有关。肾脏H4R的高表达可能影响HSP的病程及预后。Objective In order to detect the expression of histamine H4 receptor （H4R） in peripheral blood mononuclear cells and kidney of HSP Patients and to explore its role in the pathogenesis of HSP. Methods RT-PCR was employed to detect the expression of H4R mRNA in peripheral blood mononuclear ceils from 126 patients with HSP and 96 healthy controls. Immunohistochemical staining was employed to detect the expression of H4R renal tissue in 15 HSP patients and 6 healthy controls. Results The level of H4R mRNA from peripheral blood mononuclear cells in HSP patients was significantly higher than that in controls （P〈0.01）. The level of H4R mRNA from PBMC in the level of H4R mRNA from peripheral blood mononuclear cells in HSP patients was significantly higher than skin group and non-renal involved group （P〈0.01）. H4R mainly expressed in the human renal tubules of renal involved group, and the positive rate was 86%. While the positive rate of controls were 0.00%（P〈0.01）. Conclusion H4R may be involved in the acute phase of HSP and associated with the renal injury. The H4R high expression of renal may affect the prognosis of HSP.

关 键 词：组胺H4受体 过敏性紫癜 肾脏损害 

分 类 号：R729[医药卫生—儿科]