CD4^+CD25^+调节性T细胞及其细胞因子在寻常型银屑病患者的改变  

Changes of CD4^+CD25^+ Regulatory T cells and Cytokines in Psoriasis Vulgaris Patients

作　　者：赵宏丽[1] 郭永铁[1] 

机构地区：[1]天津市中医药大学第一附属医院,天津300073

出　　处：《中国中西医结合皮肤性病学杂志》2016年第3期139-141,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的探讨寻常型银屑病患者CD4^+CD25^+调节性T细胞及其细胞因子的改变。方法用流氏细胞仪直接免疫荧光法检测寻常型银屑病患者外周血CD4^+CD25^+T细胞的百分率、FoxP3+分子表达、细胞因子白细胞介素(IL)-10、转化生长因子(TGF)-β的表达。结果寻常型银屑病患者CD4^+CD25^+T细胞在进行期组、静止期组、健康对照组结果分别是4.55±1.11、5.26±1.38、6.03±1.96,CD4^+CD25^+FoxP3+T细胞在进行期组、静止期组、健康对照组结果分别是3.85±0.89、4.31±1.26、4.95±1.37。进行期患者明显低于静止期患者和健康对照组(P<0.05);而静止期患者与健康对照组比较差异无统计学意义(P>0.05)。CD4^+CD25^+IL-10+细胞、CD4^+CD25^+TGF-β+T细胞在进行期组、静止期组、健康对照组结果分别是11.57±5.64、14.49±8.26、16.35±9.98;2.65±0.97、4.23±1.55、4.83±1.84;进行期患者明显低于静止期患者和健康对照组(P<0.05);而静止期患者与健康对照组比较差异无统计学意义(P>0.05)。CD4^+CD25^+FoxP3+T细胞与PASI呈负相关(r=-0.659,P<0.05);CD4^+CD25^+IL-10+T与PASI呈负相关(r=-0.537,P<0.05);而CD4^+CD25^+TGF-β+T细胞与PASI直线相关(r=-0.184,P>0.05)。结论 CD4^+CD25^+调节性T细胞的比例以及Foxp3的表达、IL-10、TGF-β的表达明显下降,导致调节性T细胞的抑制功能缺陷,进而影响银屑病病情的发展。可以推测,调节性T细胞的数量或功能异常,均将影响银屑病的发病。Objective In order to investigate the change of CD4^＋CD25^＋ regulatory T cells and cytokines in psoriasis vulgaris. Methods The direct immunofluorescence assay was used to detect CD4^＋CD25^＋T cell, FoxP3^＋, IL-10 and TGF-β by flow cytometry. Results The percentage of CD4^＋CD25^＋T cell in active stage （4.55-＋1.11）% was lower than that in stationary stage [（5.26±1.38）%] and the control group [（6.03±1.96）%, P〈0.05]. The percentage of CD4^＋CD25^＋FoxP3^＋ Tcell in active stage were lower than that in stationary stage and control group（P〈0.05）. However, there was no statistical significant difference in stationary stage and control group （P〉0.05）. CD4^＋CD25^＋IL-10^＋ T cell and CD4^＋CD25^＋TGF-β^＋T cell decreased in active stage （P〈0.05）, but there was no statistical significance in stationary stage and the control group （P〉0.05）. There was positive relationship between CD4^＋CD25^＋FoxP3^＋ T cell and PSAI（r=-0.659, P〈0.05）. And there was positive relationship between CD25^＋IL-10^＋T cell and PSAI （r=-0.537, P〈0.05）. However, no correlation was found between CD25^＋TGF-β^＋T cell and PASI （r=-0.184, P〉0,05）. Conclusion The proportion of CD4^＋CD25^＋ regulatory T cells and the expression of Foxp3, IL-10 and TGF-β are significantly decreased. It results in the defect of inhibition function, and then influenced the development of psoriasis disease. It suggests that the abnormal of regulatory T cells number and function can influence of psoriasis pathogenesis.

关 键 词：寻常型银屑病 CD4^+CD25^+调节性T细胞 FOXP3 白细胞介素-10 转化生长因子-Β 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]