添加纳米TiO2制作的人工种皮对种子萌发的影响  

Effect of Artificial Seed Coat Containing Nano TiO_2 on Germination

作　　者：谷睿智 吴沿友[1,2] 滕博群 吴沿胜 姚香萍[1] 

机构地区：[1]江苏大学农业装备工程学院,现代农业装备与技术教育部重点实验室,江苏镇江212013 [2]中国科学院地球化学研究所环境地球化学国家重点实验室,环境生物科学和技术研究中心,贵州贵阳550002

出　　处：《北方园艺》2016年第12期20-24,共5页Northern Horticulture

基　　金：江苏省高校优势学科资助项目(苏政办[2014]37号)

摘　　要：以诸葛菜组织培养中产生的不定芽为繁殖体,海藻酸钠为包埋基质,添加纳米TiO_2制作人工种皮,观察添加不同浓度的纳米TiO_2对诸葛菜人工种子萌发和抗菌能力的影响,将纳米TiO_2与NAA以及多菌灵的作用进行对比并研究共同作用。结果表明:纳米TiO_2具有与NAA相似的生长素作用,并且显著提高萌发生根速度,二者具有协同作用;无菌条件下0.1mg·L^(-1) NAA+30g·L^(-1) TiO_2+1/2MS+3%蔗糖的组合使诸葛菜人工种子萌发率达到100%,生根率达到80%,为最佳组合;有菌条件下添加30g·L^(-1)纳米TiO_2试验组15d污染率仅为5.6%,萌发率达83.33%,为最佳组合。纳米TiO_2和多菌灵混合作用反而使萌发率和抗菌能力大幅降低。The Orychophragmus violaceus adventitious buds produced in tissue culture were used as propagules,the sodium alginate added nano TiO2 was made into artificial seed coat.The effect of different concentration of nano TiO2 on artificial seeds germination and antibacterial ability was observed,the differences between nano TiO2 with NAA or carbendazim and the interactions of them were studied.The results showed that nano TiO2 had the similar auxin action with NAA,and significantly increased the germinaton and rooting rate,and the two had a synergistic effect.Combination 0.1mg·L^-1 NAA＋30g·L^-1 TiO2＋1/2MS＋3% sucrose under sterile conditions made Orychophragmus violaceus artificial seeds＇ germination rate reached 100%and rooting rate reached 80%,was the best combination;the experimental group added 30g·L^-1 nano TiO2 under natural conditions made contamination rate reached 5.6%,germination rate reached 83.33%,was the best combination.The experimental group containing both nano TiO2 and carbendazim significantly reduced the germination rate and antibacterial ability.

关 键 词：诸葛菜 人工种子 纳米TIO2 萌发率 

分 类 号：S604.1[农业科学—园艺学]