濒危植物羽叶丁香组织培养  

Tissue Culture of Endangered Plant Syringapinnatifolia

作　　者：程明[1] 李厚华[2] 和子森 姜在民[3] 蔡靖[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]西北农林科技大学风景园林艺术学院,陕西杨凌712100 [3]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《北方园艺》2016年第12期92-96,共5页Northern Horticulture

基　　金：林业公益性行业科研专项资助项目(201204308)

摘　　要：以濒危植物羽叶丁香的芽和种子为外植体,采用植物组织培养法,研究了不同消毒方法对无菌体系建立、不同浓度的蔗糖和激素组合对种子初代培养、不同基本培养基和激素组合对增殖和生根的影响,以期为羽叶丁香的组培快繁、种质资源保护和开发利用提供依据。结果表明:新枝为较适宜的外植体,适宜的消毒方式为75%酒精消毒30s,0.1%升汞消毒7min,最适增殖培养基为MS+5.0mg·L^(-1) 6-BA+0.01mg·L^(-1) IBA;最适种子初代培养基为MS+20g·L^(-1)蔗糖汁+3.0mg·L^(-1) 6-BA+0.10mg·L^(-1) IBA,最适增殖培养基为MS+7.0mg·L^(-1) 6-BA+0.05mg·L^(-1) IBA;最适生根培养基为WPM+2.0mg·L^(-1) IBA。The buds and seeds of endangered plant Syringapinnatifolia were used as the test materials,the influence of different disinfection methods,concentration combinations of sucrose and hormone on primary culture of seeds,combinations of basal media and hormones on shoots proliferation and rooting by plant tissue culture method were studied,in order to provide basis for tissue culture propagation,germplasm resources protection and exploitation of Syringapinnatifolia.The results showed that the suitable explants were shoots.The appropriate disinfection methods were 75% ethanol for 30 seconds,0.1% mercuric chloride for 7minutes,the best proliferation medium was MS＋5.0mg·L^-1 6-BA＋0.01mg·L^-1 IBA;the optimal initial,proliferation and rooting medium for seed was MS＋20g·L^-1 sucrose＋3.0mg·L^-1 6-BA＋0.10mg·L^-1 IBA,MS＋7.0mg·L^-1 6-BA＋0.05mg·L^-1 IBA and WPM＋2.0mg·L^-1 IBA respectively according to this study.

关 键 词：羽叶丁香 组织培养 芽 种子 

分 类 号：S685.26[农业科学—观赏园艺]