新疆野扁桃自交不亲和相关基因PetSSK1转“梦幻”矮牵牛的研究  

Research on Wild Almond Self-incompatibility Related Gene PetSSK1 Transferring Into Petunia ‘Dream'

作　　者：夏江宏[1] 曾斌[1] 王建友[2] 李伟阳[1] 田嘉[1] 李疆[1] 

机构地区：[1]新疆农业大学林学与园艺学院,新疆农业大学果树学新疆特色果树研究中心,新疆乌鲁木齐830052 [2]新疆林业科学院推广站,新疆乌鲁木齐830063

出　　处：《北方园艺》2016年第12期96-100,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31260465);国家林业公益性行业科研专项资助项目(201304701-1);新疆维吾尔自治区科技计划资助项目(201130102-1-5);新疆维吾尔自治区果树学重点学科资助项目(201007)

摘　　要：以新疆野扁桃(Prunus tenella)花粉为试材,构建其自交不亲和性相关基因PetSSK1的植物表达载体pCAMBIA1303-PetSSK1,采用根癌农杆菌介导法,将该基因导入矮牵牛,并研究了不同种类及浓度抗生素对矮牵牛再生的影响。结果表明:该试验成功获得了转化新疆野扁桃自交不亲和相关基因PetSSK1的矮牵牛植株,并筛选出矮牵牛的卡那霉素(Kan)和头孢霉素(Cef)的敏感浓度分别为25、300mg·L^(-1),并进一步通过PCR分子检测确认其为转基因阳性植株。Taking the pollen of Xinjiang wild almond（Prunus tenella）as material,the expression plasmid pCAMBIA1303-PetSSK1 with self-incompatibility related gene PetSSK1 was constructed.Then it was transferred into Petunia mediated by Agrobacterium tumefaciens,and the effect of different kinds and concentration of antibiotics on Petuniaregeneration was studied.The results showed that the Petuniataking self-incompatibility related gene PetSSK1 of Xinjiang wild almond was gotten successfully.The sensitive concentration of Kan and Cef were 25 mg·L^-1 and 300 mg·L^-1,respectively.And the Petunia were further confirmed as positive plants by PCR detection.

关 键 词：野扁桃 PetSSK1 植物表达载体 根癌农杆菌介导 矮牵牛 

分 类 号：S681.603.6[农业科学—观赏园艺]