Chrysanthemum weyrichii基因组测定及rDNA定位  

Estimation of Genomic Size and Physical Location of rDNA of Chrysanthemum weyrichii

作　　者：钱双[1,2] 李昂[2] 赵鑫[2] 杨树华[2] 李名扬[1] 葛红[2] 

机构地区：[1]西南大学园艺园林学院,重庆市花卉工程技术研究中心,重庆400716 [2]中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,国家花卉改良中心,北京100081

出　　处：《北方园艺》2016年第12期101-105,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(C150302);国家‘863’计划资助项目(2011AA100208)

摘　　要：以Chrysanthemum weyrichii为试材,采用流式细胞术估算其基因组大小,同时利用染色体计数法统计了C.weyrichii的染色体条数并对其rDNA染色体定位进行了研究。结果表明:C.weyrichii基因组大小为10.02Gb,染色条数为2n=8x=72,5S rDNA杂交信号有4对位点,位于染色体的亚中部;45S rDNA杂交信号有8对位点,位于染色体的末端。该研究结果为该物种基因组分析以及菊属植物染色体进化研究提供了依据。Taking Chrysanthemum weyrichii as test material,the genome size of C.weyrichii was estimated by flow cytometry.The chromosome numbers and rDNA chromosomal location of C.weyrichii were studied by chromosome counting method and fluorescence in situ hybridization for the first time.The results showed that the genome size was 10.02 Gb,chromosome numbers were 2n=8x=72,four pairs of 5S rDNA located at submetacentric regions and eight pairs of 45S rDNA located at terminal regions were detected.This study provided a valuable basis for future genomic and cytogenetic studies in C.weyrichii.

关 键 词：CHRYSANTHEMUM weyrichii 流式细胞术 染色体 RDNA 荧光原位杂交 

分 类 号：S682.11[农业科学—观赏园艺]