接种丛枝菌根真菌的种植紫花苜蓿土壤中球囊霉素含量与PAHs去除的关系  

Correlations of Glomalin Contents and PAHs Removal in Alfalfa-vegetated Soils with Inoculation of Arbuscular Mycorrhizal Fungi

作　　者：杨振亚[1,2] 宗炯[2] 朱雪竹[2] 凌婉婷[2] 

机构地区：[1]江苏省环境科学研究院,江苏省环境工程重点实验室,江苏南京210036 [2]南京农业大学土壤有机污染控制与修复研究所,江苏南京210095

出　　处：《农业资源与环境学报》2016年第4期349-354,共6页Journal of Agricultural Resources and Environment

基　　金：国家自然科学基金(21477056,31270574);农业部公益性行业(农业)科研专项(201503107)

摘　　要：以菲和芘为多环芳烃（PAHs）代表物，以紫花苜蓿（MedicagosativaL．）为宿主植物，选用幼套球囊霉（Glomusetunicatum，Ge）、摩西球囊霉（Glomusgnosseae，Gm）和层状球囊霉（Glomuslamellosum，Gla）3种丛枝菌根真菌（AMF），研究了接种AMF下土壤中AMF菌丝密度、球囊霉素含量与PAHs去除率的关系。35—75d，接种Ge、Gm、Gla处理的土壤中菌丝密度、总球囊霉素含量、易提取球囊霉素含量均随时间延长而显著增大，与不接种对照相比，75d时接种Ge、Gm、Gla处理的土壤中易提取球囊霉素含量提高了48．58％、55．99％和50．23％，总球囊霉素含量则提高了38．75％、50．95％和46．12％。接种AMF促进了土壤中菲和芘的去除，随着时间（35—75d）延长，接种Ge、Gm、Gla处理的土壤中菲去除率分别高达83．4％-92．7％、82．1％-93．8％、86．9％-93．4％，芘去除率达42．2％-63．5％、43．7％-69．2％、44．6％-66．4％。接种Ge、Gm和Gla处理土壤中AMF菌丝密度、总球囊霉素含量均与土壤中菲和芘的去除率之间存在极显著正相关关系，表明接种AMF提高了土壤中AMF菌丝密度和总球囊霉素含量，并促进了土壤中PAHs的去除。研究结果为阐明丛枝菌根修复PAHs污染土壤的规律及机理提供了依据。The correlations of glomalin contents and removal of phenanthrene and pyrene as representative polycyclic aromatic hydrocarbons （PAHs） in soils with inoculation of arbuscular mycorrhizal fungi（AMF） were investigated. The test AMF included Glomus e tunicatum（ Ge ）, Glomus rnosseae （Gm）, and Glomus lamellosum（Gla）, and the host plant was alfalfa（Medicago sativa L. ）. The AMF hyphal density and contents of easily extractable glomalin and total glomalln in AMF-inoculated soils were observed to increase with cultivation time from 35 d to 75 d. Comparing with the control treatment（CK） without AMF inoculation, the contents of easily extractable glomalin in soil increased 48.58%, 55.99% and 50.23%, and total glomalin contents increased 38.75%, 50.95% and 46.12% with Ge, Gm and Gla inoculation after 75 d, re- spectively. AMF inoculation promoted the removal of test PAHs in soils. The removal rates of phenanthrene and pyrene in soils with AMF en- hanced in 35-75 d. 83.4%-92.7%, 82.1%-93.8% and 86.9%-93.4% of phenanthrene and 42.2%-63.5%, 43.7%-69.2% and 44.6%-66.4% of pyrene in soils with Ge, Gin, Gla were removed in 75 d respectively. AMF hyphal density and total glomalin contents were observed to be significantly positively correlated with the removal rates of PAHs in soils, indicating that the colonization of AMF enhanced hyphal density and total glomalin contents and thus promoted the degradation of PAHs in the soll environments.

关 键 词：丛枝菌根真菌(AMF) 菌丝密度 球囊霉素 多环芳烃 土壤修复 

分 类 号：X53[环境科学与工程—环境工程]