FBXO39基因对肿瘤细胞代谢分子的影响  

Effect of FBXO39 Gene on Metabolic Molecules in Tumor Cells

作　　者：刘智勇[1] 上官聪 吴道兵[1] 刘晓雯[1] 赵婷[1] 李丹[1] 

机构地区：[1]湖南大学生物学院,中国湖南长沙410082

出　　处：《生命科学研究》2016年第3期202-207,共6页Life Science Research

基　　金：国家自然科学基金资助项目(81270735;81571494)

摘　　要：FBXO39是F-box蛋白家族中的一员,研究表明该蛋白是一个新的候选肿瘤-睾丸抗原,可能与人类肿瘤的发生密切相关。为了探讨FBXO39基因的生物学功能,实验中首先检测了FBXO39在不同肿瘤细胞系中的表达水平,并通过分子克隆技术构建了FBXO39的真核表达载体,进而利用高通量的PCR array代谢组学分析方法在低表达FBXO39的MCF7细胞中筛选受FBXO39调节的代谢相关基因,最后采用定量PC R实验在低表达FBXO39的Ntera2细胞中验证筛选出来的代谢基因。结果显示,在MCF7和Ntera2肿瘤细胞中,6个代谢基因(ALDH9A1、MCT1、DLAT、HMGCS、FASN、GLUL)的表达受FBXO39的正向调控,表明FBXO39可能通过上调这些基因的表达参与肿瘤细胞的代谢过程。FBXO39 is a member of the F-box protein family, and studies have shown that the protein is a newly identified cancer-testis antigen and closely related to tumorigenesis. In order to understand the biological function of FBX039 gene, the expression level of FBX039 in different tumor cell lines were detected and the recombinant eukaryotic expression vector was constructed. Then high throughput PCR array was performed to study the connection between FBX039 and metabolism-related genes in MCF7 cells （low expression of FBX039）. And the selected genes were further verified in Ntera2 cells （low expression of FBX039）. The results showed that the expression of six selected metabolism-related genes （A LDH9A 1, MCT1, DLA T, HMGCS, FASN, GLUL） was positive related to that of FBX039 gene, which indicated that FBX039 gene may play an important role in tumor metabolism.

关 键 词：F-BOX蛋白 FBXO39基因 真核表达载体 肿瘤代谢 PCR ARRAY 

分 类 号：Q756[生物学—分子生物学]