濮阳市首次柯萨奇病毒A6实验室检测  

The first laboratory testing of Coxsackievirus A6 in Puyang

作　　者：张清杰[1] 徐国锋[1] 张玉华[1] 谢为民[1] 朱文刚[1] 卫海燕[2] 

机构地区：[1]濮阳市疾病预防控制中心,河南濮阳457000 [2]河南省疾病预防控制中心,河南郑州450016

出　　处：《中国卫生检验杂志》2016年第12期1708-1710,共3页Chinese Journal of Health Laboratory Technology

基　　金：濮阳市科技局项目(130642)

摘　　要：目的建立实时荧光定量逆转录聚合酶链式反应(real-time RT PCR)法对手足口病病例标本进行柯萨奇病毒A6(CoxA6)病原检测,明确病原,为手足口病的防治对策提供依据。方法用real-time RT-PCR法对2015年5月-6月份手足口病标本进行EV(总肠道病毒)、EV71、CoxA16核酸检测,对其他肠道病毒(非EV71和非CoxA6)阳性标本再进行CoxA6核酸检测,选取CoxA6核酸1份阴性和3份阳性提取物,送河南省疾病预防控制中心复核验证检测的准确性。结果 167份标本中EV71阳性17份,CoxA16阳性13份,其他肠道病毒阳性61份;4份CoxA6核酸省级验证结果一致。结论 CoxA6是濮阳市手足口病的又一主要病原,在普通和重症病例中都存在,real-time RT-PCR法用于CoxA6检测能为临床诊断和疾病预防控制提供可靠数据。Objective To detect the Coxsackievirus A6（ CoxA6） pathogen in foot and mouth disease diseases（ HFMD） specimens by establishing real time RT- PCR method,so as to clear pathogens and provide basis for establishing countermeasures on HFMD. Methods The EV（ total intestinal virus）,EV71,CoxA16 nucleic acid of foot and mouth disease specimens from May2015 to June in 2015 were detected by using real time RT- PCR method,and then other positive enteroviruses（ non EV71 and non CoxA6） samples were tested for CoxA6 nucleic acid. 1 negative extract and 3 positive extracts were selected and sent to Henan Provincial Center for Disease Control and Prevention for review the accuracy of verification detection. Results Among167 specimens,17 were positive EV71,13 were positive CoxA16,61 were other enterovirus（ 53 parts were CoxA6）. 4 cases of CoxA6 nucleic acid were consistent with provincial verification results. Conclusion CoxA6 was another major pathogen of HFMD in Puyang. It existed in general and severe cases. Real time RT- PCR method for the detection of CoxA6 could provide reliable data for clinical diagnosis and disease control,prevention.

关 键 词：手足口病 核酸检测 柯萨奇病毒A6 

分 类 号：R725.1[医药卫生—儿科]