苯并(a)芘对人群尿1-OH-Py及外周血CYP1A1 mRNA的影响  

Impacts of benzo(a)pyrene exposure on urine 1-OH-Py and peripheral blood CYP1A1 mRNA levels of occupational crowd

作　　者：吴苗[1] 李新[2] 张慧涛[1] 聂会祥 杨瑾[1] 张红梅[1] 

机构地区：[1]山西医科大学公共卫生学院劳动卫生与环境卫生教研室,山西太原030001 [2]太原钢铁集团有限公司总医院,山西太原030003

出　　处：《中国热带医学》2016年第6期612-614,共3页China Tropical Medicine

基　　金：国家自然科学基金资助项目(No.81001285);太原钢铁集团卫生处资助项目(No.2014)

摘　　要：目的研究苯并(a)芘[Benzo(a)pyrene,B(a)P]暴露对职业人群尿中1-羟基芘(1-OH-Py)及外周血CYP1A1mRNA水平的影响,探讨两者之间的关系。方法选取118名焦化厂车间作业工人接触组和63名非职业接触人群为对照组进行问卷调查;收集研究对象工作班后6h尿样,晨起空腹抽取静脉血。用高效液相色谱法-荧光检测器法检测尿中1-OH-Py的含量,QT-PCR检测外周血细胞中CYP1A1 mRNA水平。结果接触组1-OH-Py为(0.623±0.762)μmol/mol Cr,对照组为(0.272±0.231)μmol/mol Cr,差异有统计学意义(P<0.05)。接触组CYP1A1水平为0.309±0.552,对照组为0.479±0.586,差异有统计学意义(P<0.05)。尿1-OH-Py水平与外周血CYP1A1的基因表达水平之间呈负相关(r=-0.219,P=0.017)。结论尿中1-OH-Py的水平可以作为职业暴露人群的外暴露生物标志物,接触B(a)P的职业人群外周血CYP1A1 mRNA表达水平降低,且两者之间有相关性。Objective To study the effects of occupational exposure to benzo（a）pyrene on 1-hydroxy pyrene（1-OH-Py）level in urine and CYP1A1 mRNA expression level in peripheral blood. Methods A questionnaire survey was performed to118 coking plant workshop workers（as a contact group） and 63 water supply workers（as a control group）, and their urinesamples after work 6 hours and morning fasting elbow venous blood samples were collected. The urine 1-OH-Py level wasdetected by the high performance liquid chromatography（HPLC） method, and the mRNA of CYP1A1 expression level inperipheral blood cells was detected by QT-PCR. Results The mean levels of 1-OH-Py were（0.623±0.762） μmol/mol Cr inthe contact group and（0.272±0.231） μmol/mol Cr in the control group respectively, and there was a significant differencebetween the two groups（P〈0.05）. The levels of CYP1A1 were（0.309±0.552） in the contact group and（0.479±0.586） in thecontrol group respectively, and there was a significant difference between the two groups（P〈0.05）. The urine 1-OH-Py leveland CYP1A1 gene expression of peripheral blood cells showed a negative correlation（r =-0.219, P = 0.017）. Conclusion The 1-OH-Py level in urine can be used as a biomarker of outside to occupational exposure population, and the peripheralblood CYP1A1 mRNA expression level of the contact benzo（a）pyrene occupational population is reduced, and they are relatedwith each other.

关 键 词：苯并(A)芘 1-羟基芘 外周血 CYP1A1 MRNA 职业人群 

分 类 号：R135[医药卫生—劳动卫生]