锰超氧化物歧化酶基因变异与血脂和同型半胱氨酸水平的关系研究  

Study on the Relationship between Manganese Superoxide Dismutase Gene Variants and the Levels of Blood Lipid and Homocysteine

作　　者：赵佳[1] 左林[2] 姚创利[1] 黎阳[1] 姜小建[1] 杨军乐[3] 

机构地区：[1] 西安市中心医院 检验科,西安,710003 [2] 西安市中心医院 放射科,西安,710003 [3] 第四军医大学唐都医院放射科,西安,710038

出　　处：《现代检验医学杂志》2016年第3期12-15,共4页Journal of Modern Laboratory Medicine

基　　金：陕西省科学技术研究发展计划项目(项目编号:2013K12-02-10)

摘　　要：目的探讨锰超氧化物歧化酶（Mn-SOD）9 Ala／Val基因多态性与血脂和同型半胱氨酸（HCY）水平的关系。方法应用测序法检测137例冠心病患者和85例对照组的 Mn-SOD 9 Ala／Val基因多态性的基因型，应用比色法检测血清总超氧化物歧化酶（T-SOD）和 Mn-SOD活性，应用终点法测定血清胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）水平，应用酶法测定血清 HCY水平。结果冠心病组的Mn-SOD 9 VV基因型和V等位基因频率显著高于对照组。冠心病组的T-SOD和 Mn-SOD活性显著低于对照组。Mn-SOD 9 VV基因型的 T-SOD和 Mn-SOD活性显著低于 Mn-SOD 9 AA基因型。Mn-SOD 9 VV基因型的血清 TC，TG，LDL-C和 HCY水平显著高于 Mn-SOD 9 AA基因型，HDL-C水平显著低于Mn-SOD 9 AA基因型。Mn-SOD活性与血清TC，TG，LDL-C和 HCY水平呈负相关，与血清 HDL-C水平呈正相关。结论冠心病患者机体的抗氧化能力降低。Mn-SOD 9 Ala／Val基因多态性通过影响 Mn-SOD活性进而导致血脂代谢紊乱，促进冠心病的发生发展。HCY通过自身氧化和抑制 Mn-SOD活性，致使体内氧化物质增多，增加冠心病的发病风险。Objective To investigate the relationship between manganese superoxide dismutase （Mn-SOD）9 Ala/Val genetic polymorphisms and the levels of blood lipid and homocysteine （HCY）.Methods The genotypes of Mn-SOD 9 Ala/Val ge-netic polymorphisms were identified by sequencing method,the serum activities of T-SOD and Mn-SOD were detected by colorimetric method,the serum level of HCY was detected by enzymatic method,and the serum levels of cholesterol （TC）, triglyceride （TG）,high density lipoprotein cholesterol （HDL-C）and low density lipoprotein cholesterol （LDL-C）were de-tected by end-point method in 137 patients with coronary heart disease （CHD）and 85 controls.Results Compared with the control group,the VV genotype and V allele of Mn-SOD 9 Ala/Val genetic polymorphisms in the CHD group were higher, while the serum activities of T-SOD and Mn-SOD in the CHD group was significantly lower.The serum activities of T-SOD and Mn-SOD of the Mn-SOD 9 VV genotype was significantly lower than the Mn-SOD 9 AA genotype.Compared with the Mn-SOD 9 AA genotype,the serum levels of TC,TG,LDL-C and HCY of the Mn-SOD 9 VV genotype were significantly higher,while the serum level of HDL-C was significantly lower.The serum activity of Mn-SOD was negativelycorrelated with the serum levels of TC,TG,LDL-C and HCY and positively correlated with the serum level of HDL-C.Conclusion The antioxidative ability in patients with CHD was decreased.Mn-SOD 9 Ala/Val genetic polymorphisms led to lipid metab-olism disorders by affecting the Mn-SOD activity,promoting the development of CHD.HCY resulted in increased oxidative substances by self-oxidation and inhibition of the Mn-SOD activity,increasing the risk of CHD.

关 键 词：锰超氧化物歧化酶 基因多态性 血脂 同型半胱氨酸 冠心病 

分 类 号：Q754[生物学—分子生物学] R446.12[医药卫生—诊断学]