类风湿关节炎患者滑膜抗环瓜氨酸肽表位表达与肽酰基精氨酸脱亚氨酶4基因的相关性分析  

Relation Analysis of Synovial Anti-Citrullinated Epitope Peptide Expression and Peptidyl Arginine Deiminase4 Gene in the Rheumatoid Arthritis Patient

作　　者：沈小辉[1] 喻伟[1] 杨菲[1] 

机构地区：[1]湖北医药学院附属东风医院创伤外科

出　　处：《现代检验医学杂志》2016年第3期26-29,共4页Journal of Modern Laboratory Medicine

基　　金：湖北省自然科学基金项目(编号:2012FFB02002),新型多孔BG/CS支架材料复合TAZ基因转染BMSCs修复骨缺损的实验研究

摘　　要：目的探讨类风湿关节炎患者滑膜抗环瓜氨酸肽（CCP）表位表达与肽酰基精氨酸脱亚氨酶4（PADI4）基因的相关性。方法2013年2月～2015年选择在湖北医药学院附属东风医院风湿免疫科住院的类风湿关节炎患者110例作为观察组，同期选择在该院进行体检的健康者110例作为对照组，两组都进行滑膜抗 CCP表位表达阳性率检测与 PADI4基因表达检测，同时进行相关性分析。结果观察组与对照组的滑膜抗 CCP表位表达阳性率分别为70.9％和9.1％，观察组明显高于对照组（χ2＝23.289，P＜0.05）。观察组 PADI4-104基因型的表达频率与对照组对比差异有统计学意义（χ2＝2.984，P＜0.05），多为 G／G表达，而对照组多为C／G表达。Pearson相关性分析显示在观察组中，滑膜抗 CCP表位阳性表达情况与PADI4-104基因型表达频率之间存在明显相关性（r＝0.344，P＜0.05）；logistic逐步回归分析显示 PADI4-104基因型表达频率是影响滑膜抗CCP表位表达的主要因素（P＜0.05）。结论类风湿关节炎患者的滑膜抗 CCP表位表达阳性率明显增加，同时也存在PADI4基因多态性紊乱情况，两者也存在明显的相关性，从而影响类风湿关节炎的发病状况。Objective To investigate the relation of synovial anti-citrullinated epitope peptide（CCP）expression and peptidyl arginine deiminase 4 （PADI4）gene in the rheumatoid arthritis patient.Methods From February 2013 to 2015 in Dongfeng General Hospital Affiliated to Hubei University,selected 110 patients with rheumatoid arthritis as the observation group, and selected 110 healthy people at the same period in this hospital for medical examination as the control group,both groups were given the synovial CCP expression positive rate and PADI4 gene expression detecting and correlation analysis.Results The synovial anti-CCP expression positive rates in the observation group and the control group were 70.9% and 9.1% re-spectively,and the observation group were significantly higher than the control group （P〈0.05）.The PADI4-104 genotype expression frequency compared between the observation group and control group,the difference were statistically significant （P〈0.05）.The G/G expression was more in the observation group,while the control group was more wit the C/G expres-sion.Pearson correlation analysis showed that in the observation group,synovial CCP positive expression were significant correlated to the expression of genotype PADI4-104 （r=0.344,P〈0.05）.Logistic regression analysis showed PADI4-104 genotype frequency were the main factors for the synovial CCP epitope expression （P〈0.05）.Conclusion The rheumatoid arthritis was more with synovial anti-CCP positive expression.There were also PADI4 polymorphism disorder expression, and clear correlation between the two.Thus impact the incidence of rheumatoid arthritis.

关 键 词：类风湿关节炎 滑膜抗环瓜氨酸肽 肽酰基精氨酸脱亚氨酶4 相关性 peptidyl ARGININE deiminase 4 

分 类 号：R593.22[医药卫生—内科学] R392.1[医药卫生—临床医学]