冠心病患者α-内收蛋白基因多态性与表达水平研究  

Study on Polymorphisms and Genes Expression Levels of a-Adducin in Patients with Coronary Artery Disease

作　　者：张阳东 陈洁 楚瑞雪 王利营 云洁 安娟 方靓 

机构地区：[1] 火箭军总医院 特勤医学科,北京,100088 [2] 火箭军总医院 护理部,北京,100088 [3] 火箭军礼士路门诊部,北京,100820

出　　处：《现代检验医学杂志》2016年第3期59-61,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨α-内收蛋白基因（ADD1）的单核苷酸多态性（SNP）及表达水平与冠心病（CAD）的相关性。方法提取2011年1月～2013年10月就诊于火箭军总医院的114例CAD患者和116例健康对照者外周血白细胞DNA和RNA，应用荧光标记单碱基延伸及杂交技术检测ADD1基因的rs3775067和rs1263359多态性，应用荧光标记及毛细管电泳技术检测 ADD1的基因表达水平。结果 ADD1基因2个 SNP的基因型频率和等位基因频率在两组间差异无统计学意义（χ2＝0.018～1.317，P 值均＞0.05）。CAD 组的 ADD1基因表达水平为0.226＆#177;0.284，明显高于健康对照组（0.153＆#177;0.144），差异有统计学意义（P＜0.05）。通过对不同基因型间表达水平的比较，结果显示 CAD组 rs3775067的 TC型患者的基因表达水平（0.250＆#177;0.319）明显高于健康对照组（0.154＆#177;0.156，P＜0.05），余各基因型两组间表达水平差异无统计学意义（t＝0.557～1.867，P 值均＞0.05）。结论 ADD1基因表达水平的升高是 CAD 的危险因素，而 rs3775067和rs1263359多态性与CAD的易感性无关。Objective To investigate the association between single nucleotide polymorphisms （SNP）and expression levels ofα-adducin（ADD1）gene in coronary artery disease （CAD）patients.Methods Extracted DNA and RNA samples of peripher-al blood white cells from 114 CAD patients and 116 healthy individuals in Jan 2011 to Oct 2013 from the General Hospital of the PLA Rocket Force.SNPs of rs3775067 and rs1263359 mutations in the ADD1 gene were analyzed with allele-specific flu-orogenic oligonucleotide probes combining hybridization.The gene expression levels were analyzed with fluorescence labeled and capillary electrophoresis technology.Results The frequencies of the genotypes and alleles of the two SNPs in the ADD1 gene were not significantly different between the two groups （χ2=0.018~1.317,all P〉0.05）.The ADD1 gene expression levels of CAD group （0.226±0.284）were obviously higher than that of control group （0.153±0.144,P〈0.05）.The gene expression levels of TC genotype of rs3775067 were obviously higher in CAD group （0.250±0.319）than that of control group （0.154＆#177;0.156,P〈0.05）,but the levels of the other genotypes had no significant difference between the two groups （t=0.557~1.867,all 〉0.05）.Conclusion The elevated ADD1 gene expression level would be risk factor for CAD.The polymorphisms of rs3775067 and rs1263359 had no relevance with CAD susceptibility.

关 键 词：Α-内收蛋白 冠心病 单核苷酸多态性 基因表达 

分 类 号：R541.4[医药卫生—心血管疾病] Q786[医药卫生—内科学]