黄芩苷对一株产NDM-1大肠埃希菌体内外抗菌作用的研究  

Anti-bacterial Effects of Baicalin on One Escherichia coli Strain Carrying NDM-1 Gene

作　　者：刘志远[1] 潘健[1] 齐杰[1] 张婷菊[1] 马玉芝[1] 

机构地区：[1]中国中医科学院广安门医院检验科

出　　处：《现代检验医学杂志》2016年第3期124-126,共3页Journal of Modern Laboratory Medicine

基　　金：国家中医临床研究示范基地科研专项暨所级科研基金课题(2011S242)

摘　　要：目的观察黄芩苷对产 NDM-1大肠埃希菌的体内外抗菌作用。方法肉汤稀释法测定黄芩苷的最小抑菌浓度（MIC）、最小杀菌浓度（MBC）；棋盘联合药敏试验检测黄芩苷与亚胺培南的协同作用；通过小鼠菌血症模型，观察黄芩苷对感染模型的保护作用。结果黄芩苷的最小抑菌浓度和最小杀菌浓度相同，均为8 mg／ml；黄芩苷与亚胺培南呈明显的协同作用（FIC＝0.125），单独使用黄芩苷使菌血症小鼠死亡率降低25％。结论黄芩苷体内、外均具有一定的抗产 NDM-1大肠埃希菌活性。Objective To examine the bacteriostasis of baicalin against Escherichiacoli strain carrying NDM-1 gene.Methods Minimal inhibitory concentration（MIC）and minimal bactericidal concentration（MBC）were determined by broth dilution method.Synergy function was designed withcheckerboard method.Anti-infection effect in vivo of baicalin was observed in mice with bacteremia.Results In vitro antibacterial tests showed that the MIC and MBC baicalin were 8 mg/ml.Synergistic inhibitory effect was observed between baicalin and imipenem （FIC=0.125）.In vivo inhibition experiments showed that ba-icalin had decreased the mortality of 25% of Escherichiacoli Strain carrying NDM-1 gene infection in mice.Conclusion Ba-icalin has antibacterial effect on NDM-1 E.coli.

关 键 词：黄芩苷 大肠埃希菌 NDM-1 金属酶 抗菌作用 

分 类 号：R378.21[医药卫生—病原生物学] R446.5[医药卫生—基础医学]