百合在NaHCO_3胁迫下的生理响应机制  

The Physiological Response of Lily to NaHCO_3 Stress

作　　者：李雅男[1] 袁素霞[1] 徐雷锋[1] 杨盼盼[2] 冯亚言 Younes Pourbeyrami Hir 明军[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]南京林业大学风景园林学院,南京210037

出　　处：《园艺学报》2016年第6期1126-1140,共15页Acta Horticulturae Sinica

基　　金：国家科技支撑计划项目(2012BAD01B07);国家社会公益研究专项(2005DIB3J022);北京市花卉重点项目(YLHH2006001;YLHH201300101);农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：比较了耐盐碱的岷江百合(Lilium regale Wilson)和对盐碱敏感的东方百合‘索邦’(‘Sorbonne’)在不同浓度的碱性盐NaHCO_3胁迫下叶片的渗透调节物质、活性氧清除物质、离子调节物质(Na^+、K^+)及有机酸含量的变化,探讨百合耐盐碱的生理机制。研究发现,两种百合叶片中各种物质的变化趋势基本相似,但整体上岷江百合的各种物质含量或者活性均高于‘索邦’。随着NaHCO_3胁迫浓度的增加,两种百合叶片中的可溶性蛋白、脯氨酸含量增加,可溶性糖含量先升后降;SOD、GR的活性和AsA的含量先上升后下降,APX的活性和GSH的含量在低浓度胁迫时无明显变化,高浓度胁迫时显著下降;‘索邦’Na^+主要积累在成熟叶和新生叶中,岷江百合则主要积累在茎和成熟叶中,且能维持根、成熟叶和新生叶中较高水平的K^+/Na^+值;岷江百合中的草酸、乳酸和乙酸含量都一直增加或高于对照,‘索邦’中的苹果酸和乙酸含量一直增加。与‘索邦’相比,岷江百合在低浓度NaHCO_3胁迫下提高SOD活性和AsA含量,高浓度NaHCO_3胁迫下增加可溶性蛋白的含量并保持可溶性糖的稳定性,是其耐盐碱性较强的原因之一。In this experiment,osmotic regulation substances,antioxidant substances and ion were compared between Lilium regale Wilson（salt tolerance）and‘Sorbonne＇（salt sensitiveness）. The results showed that the change trend of these substances was the same in two lilies and L. regale Wilson produced more substances content than‘Sorbonne＇. Soluble protein,proline content increased with the concentration of alkaline-salt（NaHCO_3）increasing. Soluble sugar and As A content increased under lower concentration of NaHCO_3 stress,then decreased under higher concentration and the activities of SOD and GR had the same trend. The activities of APX and GSH content decreased sharply under higher concentration of NaHCO_3. Na＋ mainly accumulated in middle leaves and upper leaves in‘Sorbonne＇,but stem and middle leaves in L. regale Wilson. Middle leaves and upper leaves seemed to be main storage organs for Na＋ in‘Sorbonne＇,but stem and middle leaves in L. regale Wilson. Compared with‘Sorbonne＇,L. regale Wilson had higher K＋/Na＋ ratios in root,middle leaves and upper leaves. The content of oxalic acid,lactic acid and acetic acid in L. regale Wilson increased,as well as malic acid and acetic acid in‘Sorbonne＇. All the data indicated that compared with‘Sorbonne＇,L. regale Wilson could increase SOD activity and As A content under low concentration of NaHCO_3,increase soluble protein content and maintain established soluble sugar content under higher concentration of NaHCO_3,all of which are good explanations for its alkaline-salt tolerance.

关 键 词：百合 碱性盐胁迫 NAHCO3 渗透调节 活性氧调节 离子调节 有机酸调节 

分 类 号：S682.2[农业科学—观赏园艺]