淡竹叶颗粒中荭草苷和异荭草苷的含量测定  

Determination of Orientin and Isorientin in Danzhuye Granule

作　　者：冯琬淇[1] 李志浩[1] 瞿京红[1] 

机构地区：[1]湖北医药学院附属东风医院,湖北十堰442008

出　　处：《儿科药学杂志》2016年第7期43-46,共4页Journal of Pediatric Pharmacy

基　　金：湖北省2011协同创新中心基金;项目编号4

摘　　要：目的：建立淡竹叶颗粒中荭草苷和异荭草苷的含量测定方法。方法：采用FORTIS-C18柱（4.6 mm×250 mm,5μm）,以乙腈-0.2%磷酸溶液为流动相,采用梯度洗脱,流速1.0 m L/min,柱温30℃,检测波长330 nm。结果：荭草苷和异荭草苷分别在3.08~61.50μg/m L和2.18~43.60μg/m L浓度范围内与峰面积有良好线性关系（r=0.999 9）,平均加样回收率分别为99.1%、98.6%,RSD分别为0.94%、0.90%。结论：本方法专属性强,灵敏度高,重复性好,可作为控制淡竹叶颗粒质量的方法。Objective： To establish a method for the determination of orientin and isorientin in Danzhuye granule. Methods： The HPLC system consisted of the FORTIS-C18（ 4. 6 mm×250 mm,5 μm） column,the mobile phase consisted of acetonitrile and 0. 2%H3PO4gradient elution flow rate was 1. 0 m L / min,the column temperature was 30 ℃,the UV detector was set at 330 nm. Results： The linear response ranges were 3. 08 ~ 61. 50 μg / m L and 2. 18 ~ 43. 60 μg / m L（ r = 0. 999 9）. The average recovery of orientin and isorientin were 99. 1% and 98. 6%, RSD were 0. 94% and 0. 90% respectively. Conclusion： The method is rapid, simple,reproducible and specific. It can be an important method to control the standard of Danzhuye granule.

关 键 词：荭草苷 异荭草苷 淡竹叶颗粒 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]