尿路感染分离金黄色葡萄球菌生物膜形成能力及相关基因的分析  

Analysis of biofilm formation ability and related genes of Staphylococcus aureus isolated from urinary tract infections

作　　者：孙凤军[1] 洪海 冯伟[1] 孙艺璇[1] 夏培元[1] 

机构地区：[1]第三军医大学西南医院药剂科,重庆400038 [2]北京总后勤部郑常庄干休所卫生所,北京100141

出　　处：《重庆医学》2016年第19期2617-2619,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81373451)

摘　　要：目的探讨尿路感染分离金黄色葡萄球菌的生物膜形成能力及相关基因分布,为预防和治疗临床感染提供理论基础。方法采用标准琼脂平板倍比稀释法检测最小抑菌浓度,菌落计数法检测细菌黏附能力,96孔板结晶紫染色法检测细菌生物膜形成能力,生物膜相关基因检测采用PCR扩增法。结果 11株金黄色葡萄球菌临床株对青霉素和红霉素耐药率较高,而对万古霉素和呋喃妥因全部敏感。所有菌株都有较强的黏附能力,而生物膜形成能力一般。其中10株菌株扩增出icaAD和icaBC基因。结论尿路感染金黄色葡萄球菌的黏附能力和生物膜形成能力具有菌株差异性,ica是金黄色葡萄球菌生物膜形成的重要基因。Objective To study the biofilm formation ability and related gene distribution of Staphylococcus（S. ） aureus isolated from urinary tract infections to provide the theoretical basis for the prevention and treatment of clinical infection. Methods The minimal inhibitory concentration was detected using the agar double dilution method. The bacterial adhesion ability was determined by flat colony counting method. The biofilm formation ability was analyzed by the 96-well crystal violet staining method. The biofilmssociated genes were detected by PCR amplification. Results Eleven clinical strains of S. aureus were high resistant to penicillin and erythromycin,whereas were all sensitive to vancomycin and nitrofurantoin. All the isolates had a strong ability of adhesion,but the biofilm formation ability was weak. Among them,the icaAD and icaBC genes were amplified in 10 S. aureus isolates. Conclusion The adhesion ability and biofilm formation ability of S. aureus isolated from urinary tract infections have the strain differences,and ica is an important gene of S. aureus biofilm formation.

关 键 词：金黄色葡萄球菌 尿路感染 生物膜 黏附 ica基因 

分 类 号：R446.12[医药卫生—诊断学]