麻醉后认知功能障碍大鼠血清代谢物质的变化  

Serum metabolite changes of aged rats with post-anesthesia cognitive dysfunction

作　　者：胡蓉[1] 童建斌[1] 胡中华[1] 

机构地区：[1]中南大学湘雅三医院麻醉科,长沙410078

出　　处：《中国医师杂志》2016年第6期861-864,共4页Journal of Chinese Physician

摘　　要：目的探讨异氟醚对血清代谢物质的影响以及代谢物质与认知功能障碍的关系。方法30只SD大鼠随机分为两组：对照组（n=10）大鼠给予80％的氧气2h。异氟醚处理组（n=20）大鼠给予2％异氟醚和80％氧气2h。通过Y迷宫测定记录学会次数反映大鼠的认知功能，通过气象色谱质谱（GC／MS）来分析血清中代谢物质的变化，并观察血清中代谢物质的变化是否与认知功能的改变相关。结果异氟醚处理组大鼠学会的次数明显多于对照组[（70．75±15．30）次vs（45．40±11．21）次，P〈0．05]。与对照组相比，异氟醚处理组大鼠血清中的D-果糖、阿拉伯呋喃糖、n-正丁胺、肌醇升高，脯氨酸和丙氨酸降低（P〈0．05）。D-果糖、阿拉伯呋喃糖、n-正丁胺、肌醇和脯氨酸的变化与认知功能相关（P〈0．05）。结论血清中D-果糖、肌醇的浓度变化可以作为预测术后认知功能障碍发生和发展的标志。Objective To explore the effects of isoflurane on blood plasma metabolites （BPM） and its correlation with cognitive dysfunction. Methods Thirty female Sprague-Dawley （SD） rats were randomly divided into two groups： rats in the control group （ n = 10） received 80% oxygen for 2 hours （h） ; and rats in the isoflurane-treated group （ n = 20） were anesthetized with isoflurane and 80% oxygen for 2 h. Cognitive functions were examined using a Y-maze test to explore the learning times of rats. The level of blood plasma metabolites was detected through gas chromatography-mass spectrometry （GCMS）. Results The learning times of rats in the isoflurane-treated group was more than the learning times of rats in the con- trol group [（70.75 ±15.30） vs （45.40 ±11.21）, P 〈0.05]. D-fructose, arabinofuranose, n-butyl- amine, and inositol significantly increased （ P 〈 0. 05 ）, respectively. Whereas, L-analine and L-proline significantly decreased （ P 〈 0.05 ） in isoflurane-treated rats when compared to those in the control rats （P 〈 0.05）. Moreover, plasma concentrations of d-fructose, arabinofuranose, n-butylamine, inositol, and L-praline were positively correlated with the degree of cognitive dysfunction in the isoflurane-treated rats. Conclusions Changes of plasma concentrations of inositol , and d-fructose in aged rats are useful in predicting the occurrence and progression of post-anesthesia cognitive dysfunction.

关 键 词：麻醉/副作用 认知障碍/代谢 异氟醚/副作用 

分 类 号：R614[医药卫生—麻醉学]