瓜蒌薤白提取物中3,29-二苯甲酰基栝楼仁三醇的大鼠在体肠吸收研究  

In situ intestinal absorption of 3, 29-dibenzoyl-karounitriol in Gualou-Xiebai extract in rats

作　　者：邹纯才[1] 鄢海燕[1] 徐新颖[1] 黄馨[1] 张艳红[1] 王静 魏美玲[1] 

机构地区：[1]皖南医学院药学院,芜湖241002

出　　处：《国际药学研究杂志》2016年第3期539-542,共4页Journal of International Pharmaceutical Research

基　　金：安徽高校省级自然科学研究重大项目(KJ2015ZD41;KJ2016SD60);安徽省科技攻关项目(12010402089);国家大学生创新创业训练计划项目(201510368018;201510368018);皖南医学院重点培育基金项目(WK2014Z04)

摘　　要：目的研究瓜蒌薤白(GX)提取物中3,29-二苯甲酰基栝楼仁三醇(DK)的大鼠在体肠吸收机制。方法采用单向灌流模型,高效液相色谱-二极管阵列检测器(HPLC-PDAD)测定大鼠在体肠灌流GX提取物中DK的浓度变化,研究其吸收部位和吸收动力学特征。结果 GX提取物中DK的主要吸收部位为空肠、回肠和结肠,且三部分肠段间的吸收无显著性差异,均显著大于十二指肠的吸收(P<0.05);不同浓度的GX提取物中的DK的K_a值和P_(app)值无显著差异。结论 GX提取物中DK在全肠道有不同程度的吸收,其中空肠、回肠和结肠吸收最好,药物浓度对GX提取物中DK的K_a值和P_(app)值无影响,其吸收机制为被动扩散。Objective To study the in situ intestinal absorption behaviors of 3,29-dibenzoyl-karounitriol（DK）in GualouXiebai（GX）extract in rats. Methods A rat in situ single-pass perfusion model was used and the concentrations of the perfusate were determined by HPLC-PDAD to investigate the intestinal absorption site and mechanism. Results The main absorption site of DK in GX extract was jejunum,ileum and colon,and the absorption had no significant difference in the three different segments of rat intestine（P0.05）,but was significantly higher than that in duodenum（P0.05）. The Kaand Pappof DK had no significant difference in different concentrations of GX extract（P0.05）. Conclusion DK in GX extract could be absorbed in whole intestinal segment,with the best intestinal absorption site of jejunum,ileum and colon. Its absorbing mechanism may be related to passive diffusion.

关 键 词：瓜蒌薤白提取物 3 29-二苯甲酰基栝楼仁三醇 单向灌流法 在体肠吸收 

分 类 号：R285.5[医药卫生—中药学]