桐花树叶乙酸乙酯部位抗氧化、抗过敏活性组分的筛选  

Screening of the Antioxidant and Antiallergic Active Fractions of Ethyl Acetate Extract fromAegiceras corniculatum Leaves

作　　者：黄建新[1] 黄木水生 黄晓冬[2] 董文云 蔡建秀[2] 

机构地区：[1]福建医科大学附属第二医院,福建泉州362000 [2]泉州师范学院近海资源生物技术福建省高校重点实验室,福建泉州362000

出　　处：《泉州师范学院学报》2016年第2期1-6,共6页Journal of Quanzhou Normal University

基　　金：福建省自然科学基金项目(2015J01146);福建省教育厅科技项目(JA12278);泉州市科技计划B类项目(Z20140156);福建省(国家级)大学生创新创业计划项目资助(201510399019)

摘　　要：为了筛选桐花树叶75%乙醇提取物乙酸乙酯部位的抗氧化与抗过敏活性组分,通过硅胶柱层析、薄层层析分离出该部位的组分,采用DPPH自由基清除体系、还原力测定体系检测各组分的抗氧化活性,体外透明质酸酶抑制实验检测各组分的抗过敏活性.结果表明:相比于其他组分,组分Fr.3具有较强的DPPH自由基清除能力与还原力,DPPH自由基的半抑制浓度IC_(50)为0.09mg/mL,远小于人工合成抗氧化剂2,6-二叔丁基对甲酚(BHT)的IC_(50)(0.30mg/mL);还原力的半效应浓度EC50为0.25mg/mL,明显小于维生素C的EC50(0.91mg/mL).组分Fr.2、Fr.5、Fr.9具有较强的透明质酸酶抑制活性,IC_(50)分别为0.53,0.16,0.25 mg/mL,强于或等效于甘草酸二钾的IC_(50)(0.54mg/mL).可见,桐花树叶乙酸乙酯部位柱层析组分Fr.3是其强抗氧化活性组分,Fr.2、Fr.5、Fr.9组分是其强抗过敏组分.To screen the antioxidant and antiallergic active fractions of ethyl acetate extract from 75% ethanol extract of Aegiceras corniculatum leaves, the fractions were separated from ethyl acetate ex- tract by silica gel column chromatography and thin layer chromatography （TLC）,the antioxidant ac- tivities of each fraction were determined by the assays system of 1,1-diphenyl-2-picrylhydrazyl （DPPH - ） radical and reducing power measurement system, and the antiallergic activities of each fraction were determined by the ecto-suppress hyaluronidase experimentation.The results show that the component of Fr.3 has stronger DPPH radical scavenging ability and reducing power than other components,its IC50 value of DPPH radical scavenging ability is 0.09 mg/mL,which is far less than butylated hydroxy- toluene value of 0.30 mg/mL,its ECs0 value of reducing power （0.25 mg/mL） is less than that of Vit. C （0.91 mg/mL） obviously.h also is found that the components of Ft.2 ,Fr.5 ,Fr.9 have strong antial- lergic activities,with IC50 value of 0.53 mg/mL,0.16 mg/mL,0.25 mg/mL,respectively,which is bet- ter than or equivalent to dipotassium glycyrrhizinate value of 0.54 mg/mL.h is thus clear that the strong antioxidant activity component from ethyl acetate extract of A.corniculatum leaves is the com- ponent of Fr.3 ,and the antiallergic activity components are Fr.2 ,Fr.5 ,Fr.9.

关 键 词：桐花树叶 乙酸乙酯部位 组分 抗氧化 抗过敏 

分 类 号：Q946[生物学—植物学] R931.71[医药卫生—生药学]