支气管哮喘患者外周血CD4^+、CD8^+T细胞、CD4^+CD25^+调节性T细胞表达水平与肺功能的相关性研究  

Changes of peripheral blood CD4^+, CD8^+T cells, CD4^+CD25^+ regulatory T cells levels in patients with bronchial asthma and its correlation with lung function

作　　者：张莹[1] 王静[1] 

机构地区：[1]北京市房山区中医医院呼吸科,北京102400

出　　处：《中国医药导报》2016年第17期93-96,共4页China Medical Herald

基　　金：北京市科技计划课题"首都临床特色应用研究"(Z141107002514083)

摘　　要：目的探讨支气管哮喘患者外周血CD4~^+、CD8^+T细胞、CD4^+CD25^+调节性T细胞表达水平与肺功能的关系。方法收集2012年3月~2015年6月北京市房山区中医医院呼吸科收治的支气管哮喘患者106例,其中急性发作(急性发作组)患者59例,非急性发作(非急性发作组)患者47例,同时以72例健康者作为对照组。对所有入组研究对象进行肺活量(VC),一秒用力呼气容积占用力肺活量的百分比(FEV1/FVC%)及呼气流量峰值(PEF)检查,采用流式细胞仪检测外周血CD4^+、CD8^+T细胞及CD4^+CD25^+调节性T细胞水平,并对急性发作期患者CD4^+、CD8^+T细胞、CD4^+CD25^+Treg水平与肺功能的相关性进行分析。结果 1非急性发作组及急性发作组的肺功能指标与对照组比较均显著降低(P<0.05);与非急性发作组比较,急性发作组患者肺功能指标均明显偏低(P<0.05)。2非急性发作组及急性发作组患者CD4^+T细胞水平、CD4^+CD25^+Treg水平及CD4^+/CD8^+比值明显低于对照组,CD8^+T细胞水平、CD4^+CD25^+Treg/CD4^+比值显著高于对照组(P<0.05);与非急性发作组比较,急性发作组CD4^+T细胞水平、CD4^+CD25^+Treg水平及CD4^+/CD8^+比值显著下降,CD8^+T细胞水平、CD4^+CD25^+Treg/CD4^+比值显著上升(P<0.05)。3急性发作期患者外周血CD4^+T细胞水平与肺功能指标均呈正相关(r=0.47、0.50、0.45,P<0.05);CD8^+T细胞水平与肺功能指标均呈负相关(r=-0.54、-0.49、-0.56,P<0.05);CD4^+CD25^+Treg水平与肺功能指标均呈正相关(r=0.39、0.42、0.40,P<0.05)。结论 CD4^+、CD8^+T细胞及CD4^+CD25^+调节性T细胞在支气管哮喘疾病的发生、发展过程中发挥着重要作用,其水平可用来评估支气管哮喘病情的严重程度。Objective To investigate the relationship between lung function and the levels of CD4^＋, CD8^＋T cells, CD4^＋CD25^＋regulate T cells of peripheral blood in patients with bronchial asthma. Methods 106 patients with bronchial asthma in Fangshan Hospital of Traditional Chinese Medicine from March 2012 to June 2015 were selected as the observation subjects, 59 cases had acute asthma（acute asthma group） and 47 cases had non-acute asthma（non-acute asthma group）, 72 healthy human with physical examination at the same period were selected as the control group. The lung function indexes of vital capacity（VC）, forced expiratory volume in one second/forced vital capacity（FEV1/FVC%）and peak expiratory flow（PEF） of all subjects were detected. The levels of CD4^＋, CD8^＋T cells, CD4^＋CD25^＋regulatory T cells were determined by flow cytometry. The correlation between lung function and the expression levels of CD4^＋, CD8^＋T cells, CD4^＋CD25^＋regulate T cells in patients with acute asthma were analyzed. Results（1）The lung function indexes of VC, FEV1/FVC% and PEF in the non-acute asthma group and the acute asthma group were significantly lower than those in the control group（P〈0.05）; VC, FEV1/FVC% and PEF in the acute asthma group were obviously lower thanthose in the non-acute asthma group（P〈0.05）.（2）CD4^＋T cells, CD4^＋CD25^＋Treg cells and CD4^＋/CD8^＋ratio inthe non-acute asthma group and the acute asthma group were significantly lower than those in the control group, while CD8^＋T cells and CD4^＋CD25^＋Treg/CD4^＋ratio were obviously higher than those in the control group（P〈0.05）; compared with the non-acute asthma group, CD4^＋T cells, CD4^＋CD25^＋Treg cells and CD4^＋/CD8^＋ratio in the acute asthma group were significantly decreased, while CD8^＋T cells, CD4^＋CD25^＋Treg/CD4^＋ratio were significantly increased（P〈0.05）.（3）CD4^＋T cells in the acute asthma group were positively correlated with lung function parameters（r =

关 键 词：支气管哮喘 CD4^+ CD8^+ CD4^+CD25^+ T细胞 肺功能 

分 类 号：R562.25[医药卫生—呼吸系统]