荧光定量PCR技术联合白细胞介素-6检测在儿童肺炎支原体肺炎中的应用  

Application of Fluorescent Quantitative PCR Technique Combined With Interleukin-6 Detection in Children With Mycoplasma Pneumoniae Pneumonia

作　　者：徐前[1] 

机构地区：[1]天津港口医院儿科,天津300456

出　　处：《继续医学教育》2016年第6期102-104,共3页Continuing Medical Education

摘　　要：目的研究荧光定量PCR(fluorescent quantitative polymerase chain reaction,FQ-PCR)及白细胞介素-6,肺炎支原体Ig M检测在儿童支原体肺炎(MP)感染早期诊断中的作用。传统上临床诊断主要依靠血清学方法,在急性期血清学方法敏感性欠佳。方法利用荧光定量PCR检测呼吸系统受感染儿童血清中肺炎支原体(MP)的DNA含量,并与血清中MP特异性抗体(MP-Ig M)检测阳性结果比较。并对急性期和恢复期血清IL-6水平进行相关性分析。双份血清抗体有4倍以上升高或单次血清抗体滴度≥1:160作为肺炎支原体感染诊断标准。结果共检测120例疑为呼吸系统MP感染的患儿,血中MP-DNA阳性率与血清MP-Ig M的阳性率有一致性。但早期血清检测MP-DNA阳性率高于血清MP-Ig M的阳性率,差异有统计学意义(P<0.05)。MPP急性期患儿血清IL-6水平高于MPP恢复期。结论荧光定量PCR法血清检测可作为早期快速诊断儿童MP呼吸系统感染的有效方法。荧光定量PCR对于婴幼儿比颗粒凝集法有更高的诊断价值,并且操作简单,联合血清IL-6有利于早期诊断、早期治疗。Objective To comparison of the method between mycoplasma pneumoniae-specific lg M（MP-Ig M）antibody detection test and fluorescent quantitative PCR（FQ-PCR） test and interleukin-6 in the treatment of infection of mycoplasma pneumonia. Clinical diagnosis relies mainly on serology and, Serological methods lack sufficient sensitivity in the acute phase of the disease. Methods The method of ELISA was employed to detect MP-Ig M antibody in the blood of 120 cases of respiratory infection, at the same time, the DNA of serum of those patients（FQ-MP DNA） Was detected by means of PCR. Correlations between levels of IL-6 with acute MPP and levels of IL-6 with recovery MPP were analyzed in MPP group. The second antibody step up exceed 4 times or the first time≥1：160 as MP infection. Results The positive rate in the results between MPDNA and Ig M antibody was statistically significant. FQ-PCR have higher diagnosis value than particle agglutination in infant. The serum levels of IL-6 in acute MPP group were significantly higher than those of recovery MPP group. Conclusion Fluorescent quantitative PCR（PCR） test might be a kind of rapid, sensitive and specific assessment in detecting the MP infection. Combined detection of the two factors has great referential value in assessing the severity and predicting the prognosis for MPP.

关 键 词：肺炎支原体 荧光定量PCR 白细胞介素-6 儿童 呼吸道感染 

分 类 号：R375.2[医药卫生—病原生物学]