PDTC对心衰大鼠心肌细胞炎症通路的影响  

Effect of PDTC on Inflammation Pathways of Cardiac Muscle Cells in Rats Congestive Heart Failure

作　　者：牟鑫煜[1] 张寰[1] 

机构地区：[1]天津市第四中心医院心内科CCU,天津300140

出　　处：《继续医学教育》2016年第6期136-139,共4页Continuing Medical Education

基　　金：天津市卫生局科技基金资助项目(2013KZ016)

摘　　要：目的观察NF-κB抑制剂吡咯烷二硫基甲酸盐(PDTC)对大鼠心衰模型心脏核因子-κB活性的干预及炎症递质的影响。方法雄性Dahl盐敏感大鼠建立高血压-心肌肥厚-心力衰竭动物模型42只,随机分为A组(喂养正常饲料组)、B组(喂养高盐饲料组)、C组(早期加用PDTC组)、D组(高血压期加用PDTC组)、E组(心肌肥厚期12周后加用PDTC组)、F组(心肌肥厚期16周加用PDTC组)、G组(心力衰竭期加用PDTC组)共7组(n=6)。各组分别按特定时间段予以8%高盐饲养及PDTC 100 mg/kg/d腹腔内注射,统一观察不同时长的PDCT给药期对NF-κB活性、TNF-α、IL-6、MCP-1、CRP的影响。结果早期给予PDTC能更好的降低心衰大鼠心肌的炎症反应。结论 PDTC能够抑制心衰大鼠心肌的炎症反应,早期应用作用更加明显。Objective To evaluate the inhibitory effects of NF-κB inhibitor pyrrolidine dithiocar-bamate（PDTC） on the serum inflammatory mediators in hypertrophy-congestive heart failure rats. Methods The rat model of hypertension- cardiac hypertrophy-heart failure was made from 42 male Dahl salt sensitive rats. Rats were randomly divided into seven groups including group A（normal diet group）, group B（high salt diet group）, group C（NF-κB inhibition in early stage）, group D（NF-κB inhibition in hypertensive stage）, group E（NF-κB inhibition in cardiac hypertrophy stage of week 12） and group G（NF-κB inhibition in heart failure stage）. There were six rats for each group. Rats were administrated 8% high salt diet and injected PDTC 100 mg/（kg·d） intraperitoneally according to the prescribed time. Changes of NF-κB activity, tumor necrosis factor（TNF）-α, interleukin（IL）-6, monocyte chemotactic protein（MCP）-1 and C-reactive protein（CRP） were observed in different treatment time points of PDCT. Results Levels of NF-κB and proinflammatory cytokines were reduced after early administration of PDTC. Conclusion Early application of PDTC could obviously Inhibit the serum inflammatory mediators in hypertrophy-congestive heart failure rats.

关 键 词：心力衰竭 吡咯烷二硫基甲酸盐 免疫调节 

分 类 号：R392[医药卫生—免疫学]