水杨酸钠对大鼠下丘多巴胺水平影响的研究  

Effects of salicylate on dopamine levels in rat inferior colliculus

作　　者：刘俊秀[1] 刘轶君[2] 毛兰群[3] 李学佩[1] 马芙蓉[1] 

机构地区：[1]北京大学第三医院耳鼻咽喉科,北京100191 [2]华信医院急诊科,北京100016 [3]中国科学院化学研究所,北京100190

出　　处：《中华耳科学杂志》2016年第2期167-169,共3页Chinese Journal of Otology

基　　金：国家自然科学基金项目(30500556、21372020);北京市自然科学基金项目(7082100、7112144)联合资助~~

摘　　要：目的探讨听觉中枢核团下丘内多巴胺水平的变化在耳鸣发病机制中的作用。方法在水杨酸钠诱导建立的耳鸣动物模型基础上,利用微透析技术结合高效液相电化学检测方法,活体、动态研究水杨酸钠作用后大鼠下丘内多巴胺水平的变化。结果水杨酸钠引起大鼠下丘多巴胺水平显著性地降低。大约3小时后下丘内多巴胺水平降至最低,达到基础值的49±9%,然后缓慢的升高,大约5小时后恢复到基线水平。生理盐水对照组未引起任何明显的变化。结论大鼠下丘内多巴胺水平的显著性降低可能与水杨酸钠诱导的耳鸣产生有关,这些活体数据为多巴胺这一调节人们精神活动的重要递质参与耳鸣的发生提供直接的实验证据。Objective To investigate dopamine level changes in the inferior colliculus induced by salicylate and the role of dopamine in the mechanisms of tinnitus. Methods In an animal model of salicylate-induced tinnitus, we employed microdialysis combined with electrochemical methodologies to monitor changes of dopamine in rat inferior colliculus. Results Salicylate produced significant decrease in dopamine levels in the inferior colliculus, to a minimum of 49 ± 9% of baseline around 3 h post injection, followed by a return to the baseline by the end of the fifth hour. In contrast, saline did not produce any clear changes. Conclusion The decrease of dopamine in the inferior colliculus may be involved in the generation of tinnitus. Our in vivo data provide direct evidence of the important roles the dopaminergic system plays in the generation of tinnitus.

关 键 词：水杨酸钠 耳鸣 下丘 多巴胺 微透析 高效液相色谱 

分 类 号：R764.45[医药卫生—耳鼻咽喉科]