傣药腊肠树枝中1个新的三环生物碱类化合物及其细胞毒活性  

A new tricyclic alkaloid from twigs of Cassia fistula and its cytotoxicity

作　　者：王月德 周堃[1] 董伟[1] 周玲[1] 马航赢 杨艳[1] 邢欢欢[1] 周敏[1] 叶艳青[1] 胡秋芬[1] 

机构地区：[1]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500

出　　处：《中草药》2016年第10期1650-1652,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21362044;21032085);云南省高校科技创新团队资助(IRTSTYN 2014-11)

摘　　要：目的对傣药腊肠树Cassia fistula枝的化学成分进行研究。方法运用硅胶、凝胶及RP-HPLC等多种色谱技术进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从腊肠树枝95%乙醇提取物中分离鉴定了1个新的三环生物碱类化合物,命名为决明碱L(1)。生物活性测试中,其对NB4、SH-SY5Y、PC3、A549和MCF-7的IC50值分别为1.8、2.6、3.5、4.2和2.3μmol/L。结论化合物1为新化合物,且表现出明显的细胞毒活性。Objective To study the chemical constituents in the twigs of Cassia fistula. Methods The chemical constituents in the twigs of C. fistula were isolated by silica gel, Sephadex LH-20 column chromatography, and RP-HPLC methods. And their structures were elucidated by using various spectroscopic techniques, including extensive 1D and 2D NMR techniques. Results A new tricyclic alkaloid, named as cassiarin L（1） was isolated from 95% ethanol extract of the twigs of C. fistula. Conclusion Compound 1 is a new compound and it displays obvious cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 1.8, 2.6, 3.5, 4.2, and 2.3 μmol/L, respectively.

关 键 词：腊肠树 三环生物碱 细胞毒活性 决明碱L 傣药 

分 类 号：R284.1[医药卫生—中药学]