基于一测多评法研究配伍比例及pH值环境对黄连-大黄配伍后4种生物碱成分溶出的变化规律  

Study on dissolution of four alkaloid contents in compatability of Coptidis Rhizoma and Rheui Radix et Rhizoma based on compatability ratio and pH value environment by quantitative analysis of multi-components by single marker

作　　者：陈凯[1,2] 李慧[2] 王佳奇[1] 王月亮 宋明铭 丁传波[1] 刘一桐 刘文丛[1] 郑毅男[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国中医科学院中药研究所,北京100700

出　　处：《中草药》2016年第10期1709-1713,共5页Chinese Traditional and Herbal Drugs

基　　金：中国中医科学院自主选题(Z02063);2009年中医药行业科研专项(200907001-5)

摘　　要：目的研究配伍比例及p H值环境对黄连-大黄配伍后4种生物碱成分溶出的变化规律。方法在一测多评法的基础上,采用HPLC法测定小檗碱、巴马汀、黄连碱及表小檗碱的量。结果黄连-大黄合煎组及分煎合并组中4种生物碱的量及相对溶出率随着大黄比例增加均有相似的降低趋势,2组黄连与大黄配伍比例为1∶2时,4种生物碱的量及相对溶出率均达到最大值;且配伍比例与4种生物碱的量及相对溶出率无效应关系;盐酸溶液单煎黄连组p H值为5.2时,4种生物碱的量及相对溶出率达到最大值,p H值与4种生物碱的量及相对溶出率无效应关系。结论 p H值环境对黄连-大黄配伍后4种生物碱成分的量有影响,且不同比例黄连-大黄配伍后形成的p H值环境与用盐酸调配的p H值环境对生物碱成分的溶出产生的影响不一致。Objective To study the dissolution changes of four alkaloid contents in the comptability of Coptidis Rhizoma and Rhei Radix et Rhizoma based on compatability ratio and pH value environment. Methods To determine the contents of berberine, palmatine, coptisine, and epiberberine by HPLC based on the quantitative analysis of multi-components by single marker（QAMS） method. Results Contents of four kinds of alkaloids and their relative dissolution rates in the co-decoction or mixture of single decoction of Coptidis Rhizoma and Rhei Radix et Rhizoma have a similar reduction trend with the increasing of the ratio of Rhei Radix et Rhizoma. When the proportion of Coptidis Rhizoma and Rhei Radix et Rhizoma was 1∶2, the contents and their relative dissolution rates reached the maximum value, while the dispensing ratio has no effect relationship with the contents and the relative dissolution rates. When the p H value environment of the single decoction of Coptidis Rhizoma in the hydrochloric acid solution was 5.20, the p H value environment had no effect relationship with the contents of four alkaloids and relative dissolution rate. Conclusion There were influences on the contents of the four alkaloids and their relative dissolution rates in the compatability of Coptidis Rhizoma and Rhei Radix et Rhizoma. And both dispensing ratios and p H value environment in the different ratios of Coptidis Rhizoma and Rhei Radix et Rhizoma have the inconsistent influence on the alkaloids with the pH value environment by hydrochloric acid.

关 键 词：一测多评法 黄连 大黄 生物碱类成分 配伍 pH值环境 HPLC 小檗碱 巴马汀 黄连碱 表小檗碱 

分 类 号：R283.2[医药卫生—中药学]