黑水缬草神经保护活性成分研究  

Study on neuroprotective active constituents from root and rhizome of Valeriana amurensis

作　　者：王长福[1] 王秋红[1] 肖阳[1] 武立华[1] 万莹莹[1] 杨炳友[1] 王知斌[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究教育部重点实验室,黑龙江省中药天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040

出　　处：《中草药》2016年第11期1850-1855,共6页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省自然科学基金项目(H2015040);国际科技合作项目(2010DFA32440)

摘　　要：目的对黑水缬草Valeriana amurensis抗阿尔茨海默症（AD）有效部位中具有神经保护活性的成分进行筛选,以阐明黑水缬草发挥神经保护作用的药效物质基础。方法采用各种色谱方法对黑水缬草抗AD有效部位进行化学成分分离,并根据理化分析及波谱数据进行结构鉴定;利用MTT法筛选具有PC12神经保护作用的单体化合物。结果从黑水缬草抗AD有效部位中共分离鉴定了11个化合物,包括5个双环氧木脂素类化合物：（＋）-梣皮树脂醇-4,4′-二-O-β-D-双吡喃葡萄糖苷（1）、（＋）-紫丁香-4,4′-二-O-β-D-吡喃葡萄糖苷（2）、青刺尖木脂醇苷（3）、（＋）-8,8′-二羟基-松脂素-4,4′-二-O-β-D-吡喃葡萄糖苷（4）、青刺尖木脂醇（5）和6个环烯醚萜类化合物：jatamanin A（6）、7-羟基-8-（羟甲基）-4-亚甲基六氢环戊烯[并]吡喃-1（3H）-酮（7）、4-羟甲基-环戊烯[并]吡喃-7-甲醛（8）、patriscabroside III（9）、jatamanin E（10）及败酱苷（11）。对其进行体外活性研究发现25、12.5和5μmol/L的双环氧木脂素类化合物均能明显减轻Aβ1-42所致PC12细胞损伤。结论环烯醚萜类化合物6~10为该植物中首次分离得到,双环氧木脂素类化合物为黑水缬草抗AD有效部位发挥神经保护作用的部分药效物质基础。Objective To screen the active constituents from the anti-AD active fraction from the roots and rhizomes of Valeriana amurensis and to elucidate the therapeutic basis for the neuroprotective effect of the roots and rhizomes in V.amurensis.Methods The aective fraction of the roots and rhizomes in V.amurensis was separated with various chromatographic processes and the structures of the obtained compounds were identified by physicochemical analysis and various spectra data.The neuroprtective compounds on PC12 cells were screened by MTT assay.Results Eleven compounds were isolated from the roots and rhizomes of V.amurensis,including five bisepoxy lignans of（＋）-medioresinol-4,4′-di-O-β-D-glucopyranoside（1）,（＋）-syringaresinol-4,4′-di-O-β-D-glucopyranoside（2）,prinsepiol-4-O-β-Dglucopyranoside（3）,（＋）-8,8′-dihydroxy-pinoresinol-4,4＇-di-O-β-D-glucopyranoside（4）,prinsepiol（5）,and 6 iridoids of jatamanin A（6）,7-hydroxy-8-（hydroxymethyl）-4-methylenehexahydrocyclopenta [c] pyran-1（3H）-one（7）,4-hydroxymethyl-cyclopenta [c] pyran-7-carboxaldehyde（8）,patriscabroside III（9）,jatamanin E（10）,and patrinoside（11）.The Aβ1-42-induced PC12 cells injuries were alleviated by all the bisepoxy lignans with the concentration of 25,12.5,and 5 μmol/L.Conclusion Iridoids 6—10 are isolated from the roots and rhizomes of V.amurensis for the first time.Bisepoxy lignans are the therapeutic basis of neuroprotective effect in the roots and rhizomes of V.amurensis.

关 键 词：黑水缬草 青刺尖木脂醇苷 jatamanin A patriscabroside Ⅲ jatamanin E 神经保护 阿尔茨海默症 

分 类 号：R284.1[医药卫生—中药学]