川明参种质资源遗传多样性的SRAP分析  

Genetic diversity of Chuanmingshen violaceum by SRAP markers

作　　者：杨玉霞[1] 胡平[1] 夏燕莉[1] 周先建[1] 张美[1] 曹柳[1] 郭俊霞[1] 

机构地区：[1]四川省中医药科学院,四川成都610041

出　　处：《中草药》2016年第11期1943-1949,共7页Chinese Traditional and Herbal Drugs

基　　金：四川省科技厅科技支撑计划(2013SZ0114);四川省中医药管理局应用基础研究项目(2012-E-054);四川省省级公益性科研院所基本科研业务专项(A-2011N-35;A-2010N-39)

摘　　要：目的 利用SRAP分子标记技术对川明参Chuanmingshen violaceum种质资源进行遗传多样性研究,为不同来源的川明参亲缘关系及其优良种质资源的筛选提供依据。方法 采集5个不同生态区域的63份川明参种质资源,利用SRAP分子标记构建其DNA指纹图谱,从分子水平检测其遗传多样性。结果 37对SRAP引物组合共得到374条扩增条带,其中有283条呈现多态性,占75.67%。供试材料的遗传相似系数的变化范围0.726 7~0.923 9,平均值为0.815 0,整个群体的遗传相似程度差异较大。基于SRAP的聚类分析可将63份材料完全分开,并划分为7类,聚类结果与材料的地理分布有一定关系,来源于阆中和金堂的材料多样性相对较为丰富。结论 川明参种质资源在分子水平上确实存在较大遗传差异,SRAP分子标记是评价川明参资源遗传多样性的有效方法。Objective In order to correctly identify the different germplasm resources of Chuanmingshen violaceum and gain the excellent germplasm resources,SRAP molecular marker was used to analyze the genetic diversity of C.violaceum.Methods C.violaceum was collected from seven different areas,which included 24 samples,the DNA fingerprint of C.violaceum was constructed with SRAP molecular marker,and the genetic diversity was analyzed.Results Totally 374 bands were amplified by37 primer pairs,of which 283 bands were polymorphic,and the polymorphic percentage was 75.67%.The SRAP-based genetic similarity coefficient of all samples ranged from 0.726 7 to 0.923 9,with a mean of 0.815 0.The analysis of molecular variance showed higher percentages of genetic variation within population.All the accessions could be distinguished by SRAP markers.The cluster analysis results showed that 63 accessions were classified into seven groups,which were correlated with the geographical distribution of the accessions to some degree.The accessions from Langzhong and Jintang had higher diversity.Conclusion There actually exists plentiful genetic diversity among the genetic resources of C.violaceum.SRAP marker is a useful method for analyzing the genetic diversity among C.violaceum accessions.

关 键 词：川明参 SRAP分子标记 遗传多样性 聚类分析 种质资源 

分 类 号：R282.12[医药卫生—中药学]