UPLC法测定7种秦艽中7种指标成分的量  

Contents of seven indicative constituents in seven kinds of Gentiana macrophylla by UPLC

作　　者：杨燕梅[1] 马晓辉[1] 卢有媛 吕培霖[3] 林丽[1,2] 王振恒[1,2] 晋玲[1,2] 朱田田[1,2] 

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]中(藏)药资源研究所,甘肃兰州730000 [3]中国人民解放军第二十五医院,甘肃酒泉735000

出　　处：《中草药》2016年第11期1968-1973,共6页Chinese Traditional and Herbal Drugs

基　　金：中央本级重大增减支项目“名贵中药资源可持续利用能力建设”子课题“秦艽全国生产区划研究”（20603020212）;“秦艽质量特征和商品规格研究”（20603020110）;2015年甘肃省高等学校科学研究项目“濒危中（藏）药麻花秦艽遗传分子与药效成分相关性研究”（2015A-098）

摘　　要：目的 建立秦艽中马钱苷酸、獐牙菜苦苷、6′-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷和异牡荆苷测定的UPLC方法。方法 采用色谱柱ACQUITY UPLC？BEH C18（50 mm×2.1 mm,1.7μm）;流动相甲醇-0.04%磷酸水溶液梯度洗脱,体积流量0.3 m L/min;检测波长242 nm;柱温30℃。结果 马钱苷酸、獐牙菜苦苷、6′-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷和异牡荆苷分别在2.100~537.100、1.050~270.000、0.920~236.000、11.100~2 830.000、0.750~192.000、0.167~102.000、0.216~52.800μg进样量与峰面积积分值线性关系良好（r≥0.999 5）,平均回收率97.83%~100.08%,RSD≤3.76%。结论 UPLC较HPLC更简便、更有效,可用于秦艽中7种指标成分的同时测定。Objective An ultra performance liquid chromatographic（UPLC） method was developed for simultaneously determining seven constituents,such as loganic acid,swertiamarin,6′-O-β-D-glucosyl gentiopicroside,gentiopicroside,sweroside,isoorientin,and isovitexin,from the medicinal plants of Gentiana macrophylla.Methods The separation was performed on an Acquity UPLC？BEH C18 column（50 mm ×2.1 mm,1.7 μm） through a gradient elution of methanol-0.04% aqueous phosphorite at a flow rate of 0.3 mL/min.The detection wavelength was 242 nm,and the column temperature was set at 30 ℃.Results For the seven analytes,loganic acid,swertiamarin,6′-O-β-D-glucosyl gentiopicroside,gentiopicroside,sweroside,isoorientin,and sovitexin,a good linearity（r ≥ 0.9995）was obtained in the range of 2.100—537.100,1.050—270.000,0.920—236.000,11.100—2 830.000,0.750—192.000,0.167—102.000,and 0.216—52.800 μg,respectively.Their average recoveries（n = 6） were 97.83%—100.08%,respevtively,with RSD values less than or equal to 3.76%.Conclusion The UPLC method is simpler and more effective than HPLC,and can be used for the simultaneous determination of seven indicative constituents in medicinal plants of G.macrophylla.

关 键 词：秦艽组 UPLC HPLC 马钱苷酸 獐牙菜苦苷 6′-O-β-D-葡萄糖基龙胆苦苷 龙胆苦苷 獐牙菜苷 异荭草苷 异牡荆苷 

分 类 号：R286.6[医药卫生—中药学]