3.0TASL-fMRI评价灯盏花素对大鼠对比剂急性肾损伤的保护作用  

Effect of breviscapine on contrast-induced acute kidney injury in rats assessed by 3.0T arterial spin labeling function magnetic resonance imaging

作　　者：周懂晶[1] 刘玉品[1] 陈志远[1] 汪印强 冉鹏程[1] 唐梅丽[1] 易华[2] 

机构地区：[1]广州中医药大学第二临床医学院影像科,广东广州510120 [2]广州中医药大学病理教研室,广东广州510120

出　　处：《暨南大学学报（自然科学与医学版）》2016年第3期253-258,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省中医药局科研项目(20132124);广东省科技计划项目(2013B02180070);广东省中医药管理局课题(20161084)

摘　　要：目的：应用3.0T动脉自旋标记磁共振成像技术（ASL-f MRI）评估灯盏花素对大鼠对比剂急性肾损伤（CIAKI）的保护作用.方法：将60只SD大鼠随机分为对照组和灯盏花素组,对照组于注射对比剂前、后连续注射生理盐水7 d,灯盏花素组于注射对比剂前、后连续注射灯盏花素注射液7 d（每天1次）,并且于注射对比剂前、注射对比剂后20 min、24、48、72 h和7 d共6个时间点进行3.0T ASL-f MRI扫描,测量大鼠肾脏外髓部肾脏血流量（Renal Blood Flow,RBF）值,取大鼠肾脏病理检查,HE染色后评价外髓部肾小管损伤情况,分析和比较两组RBF值以及病理损伤变化.结果：对照组大鼠肾脏外髓部RBF值注射对比剂20 min后RBF值升高,持续至48、72 h后回落至低于注射对比剂前,直至7 d组;灯盏花素组注射对比剂20 min后RBF值持续升高至72 h、7 d后回落至近注射对比剂前水平.注射对比剂后72 h灯盏花素组与对照组两者比较差异有统计学意义（P〈0.05）,余时间点两者比较差异无统计学意义（P均〉0.05）.病理结果表明对照组注射对比剂后20 min后肾脏外髓部肾小管即出现损伤,24-48 h损伤减轻,72 h损伤又加重,但低于20 min,7 d后损伤加重,与20 min组相仿;灯盏花素组注射对比剂后20 min肾脏外髓部肾小管严重损伤,24-48 h损伤减轻,逐渐低于对照组损伤程度,72 h损伤又加重,但低于对照组,7 d后损伤减轻.注射对比剂后,除24 h组两者损伤无统计学差异（P〉0.05）外,余时间组两者间差异均有统计学意义（P值均〈0.05）.结论：3.0T ASL-f MRI技术可以评估CIAKI引起的肾血流灌注水平,在一定程度上反映灯盏花素注射液对大鼠CIAKI的治疗反应.Aim：To evaluate the protective effect of breviscapine on contrast agent of acute kidney injury in rats using 3.0T arterial spin labeling magnetic resonance imaging technology （ASL）. Methods： The 60 SD rats were randomly divided into control group and breviscapine group （each n=30）respectively to the injection of contrast agent before and after continuous injection of Breviscapine and normal saline 7 days （one time everyday）,ASL-fMRI was performed on rats of each before and 20 min, 24 h, 48 h, 72 h and 7 days after injection of contrast agent, measuring rats renal outer medullary RBF（Renal Blood Flow,RBF） values, and pathological examination in kidney of rats. After HE staining evaluation outer medullary tubular damage, analyzed and compared two groups of RBF value and pathological changes.Results： Control group rats renal outer medullary RBF value injection of contrast agent after 20 min RBF values increased, continued to 48, 72 h after dropped to below before injection of contrast agents, until 7 days group, breviscapine group contrast injection 20min RBF values continued to increase to 72 hours, 7 days down to near the injection of contrast agent level. Injection of contrast agent after 72 h breviscapine group and control group the difference was statistically significant （P〈0.05）, the other difference no statistical significance （P value was 〉0.05）.Pathological results showed that control group injection of contrast agent after 20 min renal outer medullary kidney tubules damaged, reduced 24 h to 48 h after injury, 72 h increased, but less than 20 minutes, 7 days after the injury was aggravated, similar to the group of 20 min; breviscapine group injection of contrast agent after 20 min of renal outer medullary kidney canaliculus serious injury, reduce 24 h to 48 h after injury and gradually lower than that of the control group, the degree of injury, 72 h was aggravated, but lower than control group, 7 days was alleviated. After contrast injection, there was no significant diffe

关 键 词：动脉自旋标记磁共振成像技术 灯盏花素 对比剂急性肾损伤 

分 类 号：R445.2[医药卫生—影像医学与核医学]