玉竹叶斑病病原菌拮抗菌株的筛选及其发酵条件的优化  

Screening of antagonistic strains and optimization of fermentation conditions to Polygonatum odoratum leaf spot pathogen

作　　者：吕东明[1] 宫丽婷[1] 任跃英[1] 王天媛[1] 张飞飞[1] 许嘉[1] 李嘉丰 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《人参研究》2016年第3期24-27,共4页Ginseng Research

摘　　要：采用平板培养法从玉竹根茎中分离得到的18株内生真菌,通过对峙培养法,筛选出部分菌株对于供试的5种病菌均有不同的抑制作用。其中菌株DP06对玉竹叶斑病病原菌具有较强拮抗性,并对其他几种病菌均有抑制效果,通过分子鉴定,鉴定该菌株为尖孢镰刀菌(Fusarium oxysporum)。通过Design-expert软件进行单因素方差分析,利用Box-behnken法进行响应面优化,确定了其最佳发酵条件为培养时间13.29d,温度24.55℃,转速120rpm,抑菌活性达到了82.25%。By plate culture isolated 18 endophytic fungi from the roots of Polygonatum odoratum,by confrontation culture,screening test for some strains of five kinds of Pathogens have different degrees of inhibition.Wherein the strain DP06 to the leaf spot pathogen of Polygonatum odoratum has strong antagonistic,and several other pathogen also have inhibitory effect,through the molecular identification,this strain was identified of Fusarium oxysporum.Through the Design-expert carry out single factor analysis of variance,use Box-behnken method take response surface,determine the optimum fermentation conditions is：Training time 13.29 days,temperature 24.55℃,Rotating speed120 rpm,antibacterial activity reached 82.25%。

关 键 词：玉竹 分子鉴定 拮抗 响应面法 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]