熊果酸对非酒精性脂肪肝大鼠肝脏的保护作用及机制  

Study on the protective effect of ursolic acid on nonalcoholic fatty liver in rats

作　　者：高敬国[1] 

机构地区：[1]邢台市人民医院消化内科,邢台054031

出　　处：《中药药理与临床》2016年第2期27-31,共5页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究熊果酸(Ursolic Acid,UA)对非酒精性脂肪肝大鼠肝脏的保护作用及其机制。方法:取120只大鼠随机分为正常对照组、模型组,熊果酸(10、20、40mg/kg)组和多烯磷脂酰胆碱(64mg/kg)组;采用高脂饲料喂养8周的方法制备非酒精性脂肪肝大鼠模型;造模过程灌胃给药,每天1次。8周后,计算肝脏指数;测定血清中转氨酶(ALT、AST)、胆碱酯酶(CHE)含量以及血脂(TC、TG、LDL-C、HDL-C)和游离脂肪酸(FFA)水平;测定肝脏组织中TC和TG含量;ELISA法测定血清中炎症细胞因子(TNF-α、IL-6)水平;HE染色法观察肝脏组织结构形态学变化并行肝组织脂肪变性分级。结果:与模型组比较,熊果酸(20、40mg/kg)组大鼠肝脏指数显著降低,血清中ALT、AST、CHE含量和TC、TG、LDL-C、FFA水平显著降低,肝组织中TC和TG含量显著降低,血清中TNF-α、IL-6水平显著降低;熊果酸40mg/kg组HDL-C含量显著升高;熊果酸治疗组大鼠肝脏组织病变以及肝细胞脂肪性病变、炎症病变均明显改善,以熊果酸40mg/kg组效果最为显著,熊果酸(20、40mg/kg)组脂肪性病变和炎症病变程度等级较模型组均显著降低。结论:熊果酸对非酒精性脂肪肝大鼠肝脏组织具有保护作用;作用机制可能与熊果酸能够有效调节血脂,降低炎症因子水平、抑制炎症反应有关。Objective： To investigate the protective effects and mechanism of Ursolic Acid（ UA） on nonalcoholic fatty liver in rats. Methods： One hundred and twenty experimental rats were randomly devided into six groups： normal control group,model group,UA（ 10,20,40 mg / kg）groups and Polyene phosphatidylcholine 64 mg / kg group. The model rats were made by feeding with high fat dier for eightweeks,and the drugs were given by intragastric administration at the same time,once a day. Eight weeks later,the hepatic index were detected; the content of ALT,AST,CHE,TC,TG,LDL-C,HDL-C and FFA in serum were determined; the level of TNF-α,IL-6 in serum were also determined by ELISA; the content of TC and TG in hepatic tissue were detected; the histopathological changes of hepatic tissue was observed by HE staining,and the fatty lesions rating was evaluated. Results： Compared with the model group,the hepatic index of UA（ 20,40 mg / kg） treated groups were significantly decreased; the content of ALT,AST,CHE,TC,TG,LDL-C,FFA in serum and the content of TC,TG in hepatic tissue of UA（ 20,40 mg / kg） treated groups were significantly decresed,the content of HDL-C in UA 40 mg / kg treated group was significantly increased; the level of TNF-α,IL-6 were significantly decreased; the hepatic tissue histopathological changes of UA treated groups were significantly improved,especially the UA 40 mg / kg treated group; the fatty lesions rating of UA（ 20,40 mg / kg） treated groups were significantly decreased（ P 〈 0. 05）. Conclusion： UA had protective effects on nonalcoholic fatty liver in rats; which perhaps related to its effects of reducing cholesterol,lowering inflammatory cytokines levels.

关 键 词：熊果酸 非酒精性 脂肪肝 保护 

分 类 号：R285.5[医药卫生—中药学]