Akt信号通路参与并介导了虎杖苷对2型糖尿病大鼠糖脂代谢的影响  

Akt signaling pathway improved glucose and lipid metabolisms in type 2 diabetic rats induced by polydatin

作　　者：王雨[1] 郝洁[2] 李婕[3] 陈诚[1] 黄俊英[1] 黄河清[1,3] 

机构地区：[1]中山大学药学院药理毒理实验室,广州510006 [2]郑州大学第一附属医院药学部,郑州450052 [3]中山大学实验动物中心,广州510006

出　　处：《中药药理与临床》2016年第2期50-53,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家科技重大专项子课题(No:2014ZX09301307-008);教育部博士点基金(NO:20130171110097);广东省科技计划项目(NO:2014A020210007;2012B050300017)的资助

摘　　要：目的:探讨虎杖苷通过Akt通路调节2型糖尿病(T2DM)大鼠糖脂代谢的作用机制。方法:本研究采用高脂高糖饮食加小剂量链脲菌素(STZ)联合诱导的2型糖尿病大鼠模型,观察灌胃给予虎杖苷(75 mg/kg、150 mg/kg)后对糖尿病大鼠血糖血脂、肝脏病理形态以及肝脏组织Akt、GSK3β、GCK、LDLR的影响。结果:虎杖苷在75 mg/kg给药剂量时就能有效调节2型糖尿病大鼠的糖脂紊乱,降低模型动物空腹血糖(FBG)、糖化血清蛋白(GSP)、糖化血红蛋白(Hb A1c)、胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)水平;减轻肝脏组织病理损伤,明显激活模型动物肝脏组织的Akt蛋白表达,进而促进下游GSK-3β的磷酸化,上调GCK,LDLR的蛋白表达,与150 mg/kg剂量组没有明显差异。结论:虎杖苷可以有效调节糖脂代谢且该作用与其影响Akt信号通路密切相关。Objective： To explore the role of polydatin on glucose and lipid metabolisms in type 2 diabetic rats involving the Akt signaling pathway.Methods： Diabetic rats were induced by a high-fat and high-sugar diet with low-dose streptozoin（ STZ,30 mg / kg） and randomly divided into model,high dose of polydatin（ 150 mg / kg）,low dose of polydatin（ 75 mg / kg） and pioglitazone（ 5 mg / kg） treatment groups（ n = 8）.Another 8 normal rats were selected in control group. Each group was given the corresponding treatment for 8 weeks. The fasting blood glucose（ FBG）,glycosylated serum protein（ GSP）,glycosylated hemoglobin（ Hb A1c）,serum total cholesterol（ TC）,triglycerides（ TG）,low-density lipoprotein cholesterol（ LDL-C）,high-density lipoprotein cholesterol（ HDL-C） were measured by the commercially available kits. H＆E staining was made to analysis the histopathological changes in liver. Meanwhile,western blot assay was applied to detect the phosphorylation levels of Akt,glycogen synthase kinase-3β（ GSK-3β） and protein expression of glucokinase（ GCK） and low-density lipoprotein receptor（ LDLR）. Results： Polydatin（ 75 mg / kg） can significantly attenuate the abnormal increase of FBG,GSP,Hb A1 c levels and improved level levels of TC,TG,LDL-C in type 2 diabetic rats（ p 〈 0. 05）. Furthermore,Polydatin（ 75 mg / kg） can also protect the diabetic rats from pathological damage in liver and significantly increased phosphorylation levels of Akt and GSK-3β,then increased protein levels of GCK and LDLR in diabetic rat liver,which has no significant difference with the dosage of 150 mg / kg. Conclusion： Polydatin has obvious effect on regulating the glucose and lipid metabolisms in type 2 diabetic rats. The underlying mechanism was closely related to the Akt signaling pathway.

关 键 词：虎杖苷 2型糖尿病 糖脂代谢 AKT通路 

分 类 号：R285.5[医药卫生—中药学]