知母皂苷对AD模型大鼠学习记忆能力及磷酸化Tau蛋白和胆碱乙酰基转移酶表达的影响  

Effects of SAa B on the learning and memory ability of AD model rats and the expression of p-tau protein and choline acetyl transferase

作　　者：隋海娟[1] 马瑞国 刘卓[1] 闫恩志[1] 金英[1] 

机构地区：[1]辽宁医学院药理学教研室,锦州121001 [2]锦州奥鸿药业有限责任公司,锦州121000

出　　处：《中药药理与临床》2016年第2期71-73,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁省科学技术基金项目(2013022008)

摘　　要：目的:研究知母皂苷(SAa B)对Aβ1-42诱导的大鼠学习记忆障碍的改善作用及机制。方法:选用SD大鼠48只随机分为4组:正常对照组,Aβ1-42组,SAa B(1、2.5 mg/ml)+Aβ1-42组,每组12只,侧脑室注射给药后第2 d进行Morris水迷宫实验,第7d快速取海马CA1区,Western Blot法观察磷酸化tau(P-tau)、磷酸化GSK-3β、磷酸化Akt蛋白及胆碱乙酰基转移酶(Ch AT)蛋白表达水平。结果:脑室内注射Aβ1-42大鼠出现明显的空间学习记忆障碍,表现为逃避潜伏期和游泳路程较正常对照组明显延长,在原平台象限游泳时间占总时间的百分比明显降低,同时伴有海马P-tau表达明显增加,p-GSK-3β、p-Akt、Ch AT表达明显降低。侧脑室内注射SAa B后,与Aβ1-42组相比,SAa B(1、2.5 mg/ml)+Aβ1-42组大鼠逃避潜伏期和游泳路程明显缩短,原平台象限游泳时间占总游泳时间百分比升高;海马P-tau表达明显降低,p-GSK-3β、p-Akt、Ch AT表达明显增加。结论:侧脑室内注射知母皂苷可通过降低Ptau表达水平,提高Ch AT活性,从而减少神经元纤维缠结的形成,并可能通过调节胆碱能功能的途径发挥抗痴呆的作用。Objective： To investigate the effect of saponins from Anemarrhena asphodeloides bge（ SAa B） on Aβ1-42 induced on the learning and memory impairment in rats and the mechanisms. Methods： Forty-eight healthy male Sprague-Dawley（ SD） rats were randomly divided into four groups： control group,Aβ1-42 group,SAa B（ 1,2. 5 mg / ml） ＋ Aβ1-42 group,n = 12. After intracerebroventricular injection of medication,second days Morris water maze test. Seventh days,fast cash in hippocampal CA1 region,Western blotting were used for phosphorylation of tau（ p-tau）,p-GSK-3β,p-Akt and choline acetyltransferase protein expression. Results： Injection after of Aβ1-42 single ventricles model,compared with the normal control group,the escape latency and swimming distance increased and percentage of time spent in the target quadrant lower（ p 〈 0. 01）,the expression of P-tau in hippocampus was significantly increased,p-GSK-3β,p-Akt and Ch AT expression decreased. After intraventricular injection of SAa B,compared with the Aβ1-42 group,SAa B（ 1,2. 5 mg / ml） ＋ Aβ1-42 significantly shorter escape latency and swimming distance,increase the percentage of time spent in the target quadrant（ p 〈 0. 05,p 〈 0. 01）; The expression of P-tau significantly decreased and p-GSK-3β,p-Akt,Ch AT significantly increased in the hippocampus of AD rats. Conclusion： SAa B can reduce the expression level of P-tau and increase the activity of Ch AT,thereby reducing the formation of neuronal fiber entanglement,and may antidementia by play a role in the regulation of cholinergic function.

关 键 词：知母皂苷 阿尔茨海默病 Β淀粉样蛋白 磷酸化TAU蛋白 胆碱乙酰基转移酶 

分 类 号：R285.5[医药卫生—中药学]