参附强心丸对心肾综合征模型大鼠心肌细胞中LC3b、Bax表达的影响  

Effects of Shenfu Qiangxin Pills on the Expression of LC3b and Bax in Myocardial Cells of Rats with Cardiorenal Syndrome

作　　者：李旭[1] 王梓[1] 郝迪[1] 王蕾[1] 

机构地区：[1]天津市医药科学研究所,天津300020

出　　处：《中国药房》2016年第19期2602-2605,共4页China Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81202801)

摘　　要：目的:研究参附强心丸对心肾综合征(CRS)模型大鼠心肌细胞中自噬相关蛋白LC3b和促凋亡基因Bax表达的影响。方法:取大鼠随机分为假手术组(水)、模型组(水)、阳性对照组(卡托普利片2.3 mg/kg)和参附强心丸高、中、低剂量组[13.2、6.6、3.3g(生药)/kg],每组10只,除假手术组外其余各组大鼠采用腹主动脉缩窄合并肾脏急性缺血再灌注法复制CRS模型;术后8周开始ig相应药物,每天1次,连续4周。末次给药后24 h检测各组大鼠血浆中肌酐(Cr)、醛固酮(ALD)含量和心肌组织中LC3b、Bax蛋白表达,计算心室指数,观察心肌组织形态学变化。结果:与假手术组比较,模型组大鼠血浆中Cr、ALD含量,心室指数和心肌组织中LC3b蛋白表达均明显升高(P<0.05或P<0.01);心肌细胞出现胞浆红色缺失,心肌横纹排列紊乱,细胞间隙有纤维化加重等现象。与模型组比较,阳性对照组和参附强心丸高剂量组大鼠血浆中Cr、ALD含量(除阳性对照组)和心肌组织中LC3b蛋白表达均明显降低,心肌组织中Bax蛋白表达明显降低(P<0.05或P<0.01),心肌细胞病理变化得到改善;参附强心丸低、中剂量组大鼠的心室指数明显降低(P<0.05)。结论:参附强心丸可降低CRS模型大鼠血浆中Cr、ALD含量,抑制心肌细胞的自噬和凋亡。OBJECTIVE：To study the effects of Shenfu qiangxin（SFQX）pills on the expression of autophagy-associated protein LC3 b and pro apoptotic gene Bax in myocardial cells of rats with cardiorenal syndrome（CRS）.METHODS：Rats were randomly divided into sham operation group（water）,model group（water）,positive control group（Captopril tablets 2.3 mg/kg）and SFQX pills high-dose,medium-dose and low-dose groups [13.2,6.6,3.3 g（crude drug）/kg],with 10 rats in each group.CRS model was induced in those groups by abdominal-aortae-constriction＋acute renal ischemia reperfusion injury except for sham operation group;and they were given relevant medicine intragastrically 8 week after operation,once a day,for consecutive 4 weeks.Plasma contents of Cr and ALD,the protein expression of LC3 b and Bax in myocardial tissue of rats were detected 24 h after last medication;ventricular index was calculated,and morphological change of myocardial tissue was observed.RESULTS：Compared with sham operation group,the plasma contents of Cr and ALD,ventricular index and the protein expression of LC3 b in myocardial tissue increased significantly in model group（P〈0.05 or P〈0.01）;and myocardial cell suffered from endochylema red deletion,myocardial cross striation disorder,intercellular space fibrosis aggravation and so on.Compared with model group,the plasma contents of Cr and ALD（except for positive control group）and the protein expression of LC3 b and Bax in myocardial tissue decreased significantly in positive control group and SFQX pills high-dose group（P〈0.05 or P〈0.01）;myocardial pathological change was improved;the ventricular index decreased significantly in SFQX pills low-dose and medium-dose groups（P〈0.05）.CONCLUSIONS：SFQX pills can decrease the plasma contents of Cr and ALD,inhibit myocardial cell autophagy and apoptosis in CRS rats.

关 键 词：参附强心丸 心肾综合征 自噬相关蛋白 醛固酮 大鼠 促凋亡基因 

分 类 号：R285[医药卫生—中药学]