调经助孕胶囊对着床障碍小鼠胚泡着床位点数和雌、孕激素及其受体的影响  

Effects of Tiaojing Zhuyun Capsules on the Number of Blastocyst Implantation Sites,Estrogen/Progestogen and Their Receptors in Mice with Implantation Dysfunction

作　　者：王瑞杰[1] 李晖[2] 孙红[3] 褚玉霞[3] 

机构地区：[1]河南中医药大学第二临床医学院,郑州450002 [2]河南省中医院/河南中医药大学第二附属医院生殖医学科,郑州450002 [3]河南省中医院/河南中医药大学第二附属医院妇产科,郑州450002

出　　处：《中国药房》2016年第19期2629-2631,共3页China Pharmacy

基　　金：国家中医药管理局全国名老中医专家传承工作室建设项目;河南省教育厅科学技术研究重点项目(No.13A360579);河南中医学院博士科研基金项目(No.BSJJ2012-21)

摘　　要：目的:研究调经助孕胶囊对着床障碍小鼠胚泡着床位点数和雌、孕激素及其受体(ER、PR)的影响。方法:将48只妊娠小鼠随机分为正常对照组(生理盐水)、病理模型组(生理盐水)与调经助孕胶囊组(24 g/kg),每组16只,ig给药,每天1次,连续3 d。第4天上午9:00,除正常对照组小鼠外,剩余小鼠均sc米非司酮溶液0.1 ml复制着床障碍模型。第5天处死小鼠,观察各组小鼠胚泡着床位点数;放射免疫法测定血清雌二醇(E_2)、孕酮(P)水平;免疫组化法测定子宫内膜ER、PR蛋白表达水平。结果:妊娠第5天,病理模型组小鼠胚泡着床位点数显著少于正常对照组,血清E_2、P水平与子宫内膜ER、PR蛋白表达水平均显著低于正常对照组(P<0.05);调经助孕胶囊组小鼠胚泡着床位点数显著多于病理模型组,血清E_2、P水平与子宫内膜ER、PR水平均显著高于病理模型组(P<0.05)。结论:调经助孕胶囊可显著提高小鼠血清E_2、P水平和ER、PR水平,增加胚泡着床位点数。OBJECTIVE：To study the effects of Tiaojing zhuyun capsules on the number of blastocyst implantation,estrogen,progestogen and their receptors（ER,PR）in mice with implantation dysfunction.METHODS：48 pregnant mice were randomly divided into normal control group（normal saline）,pathological model group（normal saline） and Tiaojing zhuyun capsules group（24 g/kg）,with 16 mice in each group.They were given relevant medicine intragastrically,once a day,for consecutive 3 d.On the 4th day,those mice were given mifepristone 0.1 ml subcutaneously to induce implantation dysfunction model except for normal control group.On the 5th day,the mice were sacrificed.The number of blastocyst implantation sites was observed.The serum levels of estradiol（E_2）and progesterone（P）were determined by radioimmunoassay,and the expression of ER and PR protein were determined by immunohistochemistry.RESULTS：On the 5th day of pregnancy,the number of embryo implantation sites,serum levels of E_2 and P and the protein expression of ER and PR in pathological model group were significantly lower than in normal control group（P〈0.05）;the number of embryo implantation sites,levels of E_2 and P,the protein expression of ER and PR in Tiaojing zhuyun capsules group were significantly higher than in pathological model group（P〈0.05）.CONCLUSIONS：Tiaojing zhuyun capsules can effectively improve the serum levels of E_2 and P,the protein expression of ER and PR,and increase the number of blastocyst implantation sites in mice.

关 键 词：调经助孕胶囊 着床障碍 胚泡着床位点数 雌二醇 孕酮 雌、孕激素 小鼠 

分 类 号：R915[医药卫生—微生物与生化药学]