一种水溶性党参多糖的分离纯化及结构分析  

Purification and Structural Analysis of Polysaccharide from a Kind of Water-soluble Codonopsis pilosula

作　　者：刘章泉 姚晓东[1,2] 肖世基[1] 陈晓兰[1] 吴倩男 余兰[1] 

机构地区：[1]遵义医学院药学院,遵义563009 [2]遵义医学院院士工作站糖化学与糖生物学实验室,遵义563009

出　　处：《基因组学与应用生物学》2016年第6期1294-1299,共6页Genomics and Applied Biology

基　　金：遵义医学院博士启动资金协助项目(ZMKD2013-006);贵州省自然科学基金(QKH-LH[2014]7563)共同资助

摘　　要：采用水提醇沉法得到党参粗多糖（COP）,采用Sevag法除去蛋白成份,接着通过Sephacry1 S-200HR及Sephadex G-25凝胶柱色谱分离得到均一多糖COP-1。凝胶渗透色谱法（HPGPC）测定其纯度和平均分子量。高效液相色谱法（HPLC）确定其单糖的组成。红外光谱及核磁推测COP-1结构。结果表明COP-1平均分子量约为2.1×10~3 Da,且均由阶D-（2→1）呋喃果糖组成。Crude Codonopsis pilosula polysaccharide was gained using water extraction-alcohol precipitation,and then Sevag method was used to remove protein.The main fraction of Codonopsis pilosula polysaccharide,namely COP-1,was isolated by Sephacryl S- 200 HR and Sephadex G- 25 column chromatography.High Performance Gel Permeation Chromatography（HPGPC） was used to determinate the purity and molecular weight of COP-1.High Performance Liquid Chromatography（HPLC） was used to analyze the monosaccharide composition of COP-1.Its structure was elucidated by FT-IR and NMR（1H-NMR,13C-NMR,HMBC and HSQC）.The molecular weight of COP-1 was about 2.1×10~3 Da,and the mainly constitution of it was β-D-（2→1） fructose.

关 键 词：HPGPC 单糖组成 HPLC 多糖 党参 

分 类 号：R284[医药卫生—中药学]