垂序商陆抗坏血酸过氧化物酶(APX1)基因克隆及表达分析  

Cloning and Expression Analysis of Ascorbate Peroxidase(APX1) Gene in Phytolacca americana

作　　者：赵启红[1] 张皓[1] 刘海珍[1] 徐吉臣[1] 

机构地区：[1]北京林业大学,林木育种国家工程实验室,北京100083

出　　处：《基因组学与应用生物学》2016年第6期1479-1486,共8页Genomics and Applied Biology

基　　金：北京市自然科学基金项目(5112015)资助

摘　　要：从垂序商陆中克隆了抗坏血酸过氧化物酶基因PaAPX,CDS(coding sequence)全长为753 by,可编码250个氨基酸,预测蛋白分子质量为27.54 kD,等电点为5.39,二级结构主要由α螺旋和无规则卷曲组成。生物信息学分析显示,在氨基酸水平上PaAPX1蛋白与菠菜的同源性达到93.2%,系统进化树显示与菠菜、盐地碱蓬、甜菜等物种的亲缘性较近;实时荧光定量PCR结果显示,PaAPX1在块根、须根、茎、叶中均有表达,茎和叶中的表达量高于根连;镉胁迫处理后,PaAPX1在块根、须根中上调表达,茎和叶中下调表达,且根中的表达量高于茎和叶。The ascorbic acid peroxidase gene PaAPXl was cloned from the Phytolacca americana L.,and its complete length of CDS（coding sequence） was 753 bp,encoding 250 amino acids with a 27.54 kD predicting protein molecular weight and a 5.39 isoelectric point.Secondary structure was mainly composed of alpha helix and random coil.Bioinformatics analysis showed that PaAPX1 protein had a 93.2% homology with Spinacia oleracea APX1 protein on the level of amino acid,also had such close genetic relationship with Spinacia oleracea,Suaeda salsa and Beta vulgaris where system evolutionary tree displayed.Real-time fluorescent quantitative PCR results showed that the PaAPX1 gene expressed universally in the main root,fibrous root,stem and leaves,in which expression was higher in the stem and leaves than the root.The expression of PaA PX1 was up-regulated in the main root,fibrous root,and down-regulated in the stem and leaf through cadmium stress treatment,in which expression was higher in the root than that in the stems and leaves.

关 键 词：垂序商陆 抗坏血酸过氧化物酶 基因克隆 表达分析 

分 类 号：Q943.2[生物学—植物学]